Anti-Human IL-2 Rβ
Anti-Human IL-2 Rβ
Product No.: I-636
- -
- -
Clone 27302 Target IL-2 R beta Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-2 Receptor Beta, IL2RB, CD122, P70-75 Isotype IgG1 Applications CyTOF® , FC , IHC FFPE , N |
- -
- -
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human IL-2 Rβ (Accession # NP_000869) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830758 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-2 Rβ. A suggested method would be to stain cells expressing IL-2 Rβ with 0.25 µl per 1 - 2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:M1189. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2 - 15 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human IL-2 Rβ bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-2 Rβ (at 30 ng/ml) is 0.05-0.15 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone 27302 recognizes an epitope on human IL-2 Rβ. This antibody does not cross-react with rhIL-2 Rα, rhγc, rhIL-4 R or rhIL-6 R. Antigen Distribution CD122 is expressed on NK cells and at lower levels by T lymphocytes, B lymphocytes, monocytes, and macrophages. Background CD122 is a 70-75 kD IL-2 receptor β chain that is a type I membrane protein. CD122 is involved in T cell-mediated immune responses and its activation increases proliferation of CD8+ effector T cells. It exists in three forms with varying degrees of binding affinity with IL-2. The low affinity form is a monomer of the α subunit and has no involvement in signal transduction. The intermediate affinity form is a γ/β heterodimer and the high affinity form is an α/β/γ heterotrimer. The intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. This protein also interacts with the IL-15 receptor. Antigen DetailsPubMed References & Citations1. Leonard, WJ. et al. (1987) Science 238: 75 Technical ProtocolsCyTOF®  IHC FFPE N |
Related Products
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
