Anti-Human Ki-67 Antibody (34063)

Product No.: 34063

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Clone
G067.1
Target
Ki-67
Formats AvailableView All
Product Type
Monoclonal
Alternate Names
Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67
Isotype
Mouse IgG1
Applications
IHC

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Data

Immunohistochemistry: Detection of Ki-67 in human cervical cancer tissue with #34063 diluted 1:100-1:200.
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Recombinant human Ki-67.
Product Concentration
Lot Specific
Formulation
Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.
State of Matter
Liquid
Product Preparation
Purified by immunoaffinity chromatography
Storage and Handling
Store at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco


Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Mouse Monoclonal Antibody specific to Ki-67
Background
Ki-67 is a nuclear, non-histone protein that is expressed only during active phases of the cell cycle (G1, S, G2, and M), but not in the resting phases of G0 and G1 early phase. Although it has been associated with ribosomal RNA transcription, it is strongly linked to cell proliferation and has been used as an effective marker in grading proliferation rate of tumors including those of brain, breast, cervix, and prostate.
Function
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). {UniProtKB:E9PVX6, PubMed:10878551, PubMed:24867636, PubMed:27362226}.
NCBI Gene Bank ID
UniProt.org
Research Area
Cancer Research

References & Citations

Formats Available

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Prod No.
Description
34063
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.