Purified Recombinant Human MCP-4 (Accession # Q99616.1)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
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Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 µg/ml. A suitable detection antibody is PN:M127 at a concentration of approximately 100nµg/ml. A suggested standard for this assay is PN:M159.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MCP-4. A suggested method would be to stain cells expressing MCP-4 with 0.25 µg per 1 - 2.5 x 106 cells in a total staining volume of ≤ 200 µl followed by PN:M1189.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human MCP-4 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MCP-4 (at 2 ng/ml) is 10 - 30 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human Monocyte Chemotactic Protein-4 (MCP-4) (Clone 73506) recognizes an epitope on Human MCP-4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Monocyte chemoattractant protein-4 (MCP-4), also know as CCL13, is a member of a distinct, structurally-related subclass of CC chemokines mainly involved in recruitment of eosinphils to inflammatory sites. MCP-4 is a major chemoattractants for eosinophils, basophils monocytes and T lymphocytes. The MCP protein family bind to specific G-protein-coupled receptors, initiating a signal cascade within the cell1,2 MCP-4 can cause joint destruction in rheumatoid arthritis.3
References & Citations
1. Luster, AD. et al. (1996) J Immunol. 157: 5613
2. Schleimer, RP. et al. (1997) J Clin Invest. 99: 926
3. Momohara, S. et al. (2005) Rheumatology 10: 1093
Products are for research use only. Not for use in diagnostic or therapeutic procedures.