Anti-Human MD-2 (Intermediate Domain)

PN:M1224

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human MD-2 recognizes an epitope near the middle region of human, mouse and rat MD-2. This polyclonal antibody was purified using affinity chromatography.

MD-2 is a member of the Toll/interleukin-1 receptor (TIR)family, a group of proteins that include the Toll-like receptors (TLRs) (1-3). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses (4). TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS (5,6). The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4 (7).

1. O’Neill, L. et al. (2003) Trends in Imm. 24:286
2. Vogel, SN. et al. (2003) Mol. Interv. 3:466
3. Takeda, K. et al. (2003) Annu. Rev. Immunol. 21:335
4. Janeway Jr., CA. et al. (2002) Annu. Rev. Immunol. 20:197