Anti-Human HLA A2,B7 (MHC Class I) – Purified in vivo GOLD™ Functional Grade

Anti-Human HLA A2,B7 (MHC Class I) – Purified in vivo GOLD™ Functional Grade

Product No.: H1685

[product_table name="All Top" skus="H1685"]

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Clone
BB7.6
Target
HLA-A2,B7
MHC Class I
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
HLA-A, HLA-A2,B7, B.C.HLA-A2
Isotype
IgG1
Applications
FC

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Solubilized HLA-B7 antigen
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone BB7.6 recognizes an epitope shared by the human MHC class I molecule HLA-B.
Antigen Distribution
HLA-B is ubiquitously expressed on nucleated cells.
Background
HLA-B antibody, clone BB7.6, recognizes a shared epitope (Bw6) of the major histocompatibility complex (MHC) class I molecule human leukocyte antigen (HLA)-B1. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)2,3. The α-chain consists of three domains (α1, α2, and α3)4. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens5-7. β2M noncovalently associates with α3, which is essential for MHC stability. MHC class I plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation8. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling9. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance10. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity11,12.

Antigen Details

Research Area
Immunology

References & Citations

1. Lutz CT, et al. (1994) J Immunol. 153(9):4099-110.
2. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):10618-25.
3. Wieczorek M, et al. (2017) Front Immunol. 8:292.
4. Jones EY. (1997) Curr Opin Immunol. 9(1):75-9.
5. Matsumura M, et al (1992) Science. 257:927–34.10.1126/science.1323878
6. Bouvier M & Wiley DC. (1994) Science. 398–402.10.1126/science.8023162
7. Zacharias M & Springer S. (2004) Biophys J. 87:2203–14.10.1529/biophysj.104.044743
8. Cruz FM, et al. (2017) Annu Rev Immunol. 35:149-176.
9. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):16916-16921.
10. Orr MT & Lanier LL. (2010) Cell. 142(6):847-856.
11. Raulet DH. (1994) Adv Immunol. 55:381-421.
12. Salcedo M & Ljunggren HG. (1996) Chem Immunol. 64:44-58.
Flow Cytometry
Products are for research use only. Not for use in diagnostic or therapeutic procedures.