Anti-Human/Mouse/Rat β-Catenin 1 (Clone 12F7) – Biotin

Anti-Human/Mouse/Rat β-Catenin 1 (Clone 12F7) – Biotin

Product No.: C3465

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Clone
12F7
Target
Human/Mouse/Rat β-Catenin 1 (CTNNB1)
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Alternate Names
TNNB, MRD19, armadillo, catenin beta-1
Isotype
Mouse IgG1 κ
Applications
FC
,
IF
,
IHC
,
WB

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Antibody Details

Product Details

Reactive Species
Human/Mouse/Rat
Host Species
Mouse
Immunogen
N-terminal fragment of β-catenin fused to MBP
Product Concentration
0.5 mg/ml
Formulation
This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
State of Matter
Liquid
Storage and Handling
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 – 8° C Wet Ice
Additional Applications Reported In Literature ?
IHC
IF
WB
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
12F7 activity is directed against human, mouse, and rat β-catenin 1.
Background
β-catenin is an armadillo protein that binds to numerous partners, including cadherins, adenomatosis polyposis coli, and T-cell factor 1 , and is also a principal component of the Wnt signaling pathway 2 . β-catenin is necessary for embryonic development, organ differentiation and development, bone homeostasis, mammalian hair growth, and adult tissue regeneration. Additionally, β-catenin functions in cell adhesion as part of a cadherin/catenin complex, plays an important role in the structural integrity of squamous epithelial cells 3 , and is involved in synaptic plasticity 1 . β-catenin localizes to the cell cytoplasm, and once activated, translocates to the nucleus, where it activates target genes via TCF/LEF transcription factors . Abnormal signaling leads to disease, including cancer, lung disease (e.g., chronic obstructive pulmonary disease), atherosclerosis, and Alzheimer’s Disease.

12F7 was generated by immunizing Balb/c mice with a purified in-frame fusion protein of β- catenin bound to maltose binding protein 4 . The β-catenin was derived from a 2.8 kb cDNA clone isolated from a chicken embryo cDNA library, of which a 2.4 kb section encoding residue 92 through the carboxy terminus was used. Half the splenic lymphocytes were fused with the P3-X63Ag8.653 myeloma cell line and the other half with Sp2/0-Ag14. The 12F7 binding epitope was mapped to a region between residues 112 and 134 via a deletion strategy. 12F7 does not cross-react with plakoglobin.
Antigen Distribution
β-catenin 1 is widely expressed in many tissues, including kidney, lung, adult and developing central nervous system, several hair follicle cell types, colon, cortical neurons, and various cancers.
Ligand/Receptor
β-catenin localizes to cell-cell contacts (adherens junctions) and the cell membrane, as well as the cytoplasmic pool of protein.
NCBI Gene Bank ID
UniProt.org
Research Area
Cell Adhesion
.
Cell Biology
.
Neuroscience

References & Citations

1. Maguschak KA, Ressler KJ. Neuropharmacology. 62(1):78-88. 2012.
2. Liu J, Xiao Q, Xiao J, et al. Signal Transduct Target Ther. 7(1):3. 2022.
3. Politi EN, Lazaris AC, Kehriotis M, et al. Cytopathology. 19(3):172-178. 2008.
4. Johnson KR, Lewis JE, Li D, et al. Exp Cell Res. 207(2):252-260. 1993.
5. Sacco PA, McGranahan TM, Wheelock MJ, et al. J Biol Chem. 270(34):20201-20206. 1995
6. Radice GL, Rayburn H, Matsunami H, et al. Dev Biol. 181(1):64-78. 1997.
Flow Cytometry
IF
IHC
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.