Anti-Human Nectin-4 (Enfortumab)

Anti-Human Nectin-4 (Enfortumab)

Product No.: E380

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Product No.E380
Clone
AGS-22M6E
Target
Nectin-4
Product Type
Biosimilar Recombinant Human Monoclonal Antibody
Alternate Names
Enfortumab, AGS-22CE, AGS-22M, AGS-22M6E, PVRL4, anti-PVRL4
Isotype
Human IgG1κ
Applications
ELISA
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Human
FC Effector Activity
Active
Immunogen
Human Nectin-4
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This biosimilar antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Liquid
Product Preparation
Recombinant biosimilar antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade biosimilar antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 – 8° C Wet Ice
Additional Applications Reported In Literature ?
ELISA,
WB
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
AGS-22M6E activity is directed against human Nectin-4. This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Enfortumab.
Antigen Distribution
Nectin-4 is expressed in various tissues, particularly in epithelial cells. It is also expressed in certain types of cancer, making it a potential target for cancer therapeutics.
Background
Nectin-4 is a member of the Nectin family of immunoglobulin-like cellular adhesion molecules. They play a critical role in the formation and maintenance of tight junctions. These important proteins are homologs to the poliovirus receptor with Nectin-4 also known as poliovirus receptor-related protein 4 (PVRL4)1.

Nectin-4 is often overexpressed in a variety of cancers such as breast, lung, urothelial, colorectal, pancreatic, ovarian, and gastric cancers. It plays a role in cancer progression by influencing various processes such as cell proliferation, angiogenesis (formation of blood vessels) metastasis (spread to other parts of the body), and DNA repair. Given the critical role that Nectin-4 plays in cancer progression, targeting it has emerged as a promising approach for treating cancer. There have been several studies that have investigated the efficacy of Nectin-4-targeted therapies2 - 7.

AGS-22M6E (Enfortumab Vedotin) is an antibody-drug conjugate (ADC) designed to target cancer cells with high levels of Nectin-4 expression. It consists of an antibody against Nectin-4 linked to MMAE, which disrupts microtubules within the cell. Upon entering cancer cells, the ADC releases MMAE and causes cell death by disrupting the microtubule network. AGS 22M6E has received accelerated approval from the FDA for the treatment of cancer and has shown potential in clinical applications8 - 10.

Antigen Details

Ligand/Receptor
Poliovirus
NCBI Gene Bank ID
UniProt.org
Research Area
Immunology

References & Citations

1. Bouleftour W, Guillot A, Magne N. Mol Cancer Ther. 21(4):493-501. 2022.
2. Barrett JS, Gibson PR. J Am Diet Assoc. 2010;110(10):1469-1476. 2010.
3. Siddharth S, Goutam K, Das S, et al. Int J Biochem Cell Biol. 89:85-94. 2017.
4. Chatterjee S, Sinha S, Kundu CN. Eur J Pharmacol. 911:174516. 2021.
5. Liu Y, Han X, Li L, et al. Int J Oncol. 59(5):93. 2021.
6. Zhang Y, Zhang J, Shen Q, et al. Oncol Lett. 15(6):8789-8795. 2018.
7. Zhang Y, Chen P, Yin W, Ji Y, Shen Q, Ni Q. Hum Pathol. 72:107-116. 2018.
8. Challita-Eid PM, Satpayev D, Yang P, et al. Cancer Res. 76(10):3003-3013. 2016.
9. McGregor BA, Sonpavde G. Expert Opin Investig Drugs. 28(10):821-826. 2019.
10. Research C for DE and. FDA grants regular approval to enfortumab vedotin-ejfv for locally advanced or metastatic urothelial cancer. FDA. Published online July 12, 2021. Accessed January 29, 2024. https://www.fda.gov/drugs/resources-information-approved-drugs/fda-grants-regular-approval-enfortumab-vedotin-ejfv-locally-advanced-or-metastatic-urothelial-cancer
11. Penny C, Quow KL, Rundle C, et al. British Journal of Dermatology. 187. 2022.
Indirect Elisa Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.