Anti-Human Nudel

PN:N150

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human Nudel recognizes Human and Mouse Nudel. This polyclonal antibody was purified using affinity chromatography.

Nudel was initially discovered as a protein homologous to Aspergillus NUDE and that associated with Lis1 and polyprotein complex cytoplasmic dynein, both of which have been linked to neuronal development and migration.^1,2 It was later shown to be a substrate of cdk5, a kinase known to be critical during neuronal migration; phosphorylation of Nudel by cdk5 affects its localization in neurons and affects neuritic morphology.^1,3 It is thought that together with Lis1, Nudel regulates cytoplasmic dynein, a large polyprotein complex, in neuronal migration and mitosis through direct interactions.⁴ Similar interactions are thought to also play a role in membrane traffic in other cells as disruption of Nudel expression through RNA interference perturbed normal endomembrane flux and resulted in the fragmentation of the Golgi apparatus.³

1. Niethammer, H. et al. (2000) Neuron 28:697
2. Liu, Z. et al. (2000) Nat. Cell Biol. 2:776
3. Gupta, A. et al. (2003) Neurosignals 12:173
4. Liang, Y. et al. (2004) J. Cell Biol. 164:557