Anti-Human p53 Antibody (34065)

Product No.: 34065

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Clone
G053.1
Target
p53
Formats AvailableView All
Product Type
Monoclonal
Alternate Names
Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Isotype
Mouse IgG2b
Applications
IHC

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Select Product Size

Data

Immunohistochemistry: Detection of p53 in human colon carcinoma with #34065 diluted 1:100-1:200.
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Recombinant human p53.
Formulation
Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.
State of Matter
Liquid
Product Preparation
Purified by immunoaffinity chromatography
Storage and Handling
Store at 2-8°C. Do not freeze.
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco


Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Mouse Monoclonal Antibody specific to p53
Background
p53, like Retinoblastoma protein, is a tumor suppressor protein. Mutations in p53 are found in most tumor types. p53 protein binds DNA which in turn stimulates another gene to produce a protein called p21 that interacts with a cell division-stimulating protein (cdk2). When p21 is complexed with cdk2 the cell cannot pass through to the next stage of cell division. Mutant p53 can no longer bind DNA in an effective way, and, as a result, the p21 protein is not made available to act as the ‘stop signal' for cell division. Thus cells divide uncontrollably, and form tumors.
Function
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492). {PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:9840937}.
NCBI Gene Bank ID
UniProt.org
Research Area
Cancer Research

References & Citations

Formats Available

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Prod No.
Description
34065
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.