Anti-Human PDGF Rα

Product No.: P277


Clone 35248 Target PDGF R alpha Formats AvailableView All Product Type Monoclonal Antibody Alternate Names PDGF Receptor Alpha, CD140a Isotype IgG1 Applications IHC FFPE , N , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human PDGF Rα (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831573 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human PDGF Rα this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human PDGF Rα is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P253. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human PDGF Rα bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human PDGF Rα (at 10 ng/ml) is 0.2 - 0.6 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Mouse Anti-Human PDGF Rα (Clone 35248) recognizes an epitope on Human PDGF Rα. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rhPDGF Rβ or rmPDGF Rα. PubMed PDGF R alpha References & Citations View product citations for antibody P277 on CiteAb Technical Protocols IHC FFPE N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human PDGF Rα (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831573

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human PDGF Rα this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human PDGF Rα is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P253.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human PDGF Rα bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human PDGF Rα (at 10 ng/ml) is 0.2 - 0.6 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human PDGF Rα (Clone 35248) recognizes an epitope on Human PDGF Rα. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rhPDGF Rβ or rmPDGF Rα.

PubMed

PDGF R alpha

References & Citations

View product citations for antibody P277 on CiteAb

IHC FFPE

Certificate of Analysis

Request COA

Prod No.	Description
P277	Anti-Human PDGF Rα
P263	Anti-Mouse PDGF Rα
P253	Recombinant Human PDGF Rα

Formats Available

Prod No.	Description
P277	Anti-Human PDGF Rα

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Human PDGF Rα