Anti-Human Prolactin [Clone 172] — Purified

Anti-Human Prolactin [Clone 172] — Purified

Product No.: P100

[product_table name="All Top" skus="P100"]

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Clone
172
Target
Prolactin
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Mammotropin, Luterotropic Hormone (LTH), Lutetropin
Isotype
IgG

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Data

Using the antibody pair P100 and P101, the prolactin dose-response graph demonstrates strong analytical sensitivity. The curve is curvilinear and shows an estimated Limit of Detection (LoD) of ≤0.625 ng/mL from the tested samples.Prolactin LOD Graph
A strong, curvilinear dose-response for prolactin was observed using a matched antibody pair: the Leinco P100 gold conjugate and the Leinco P101 nitrocellulose membrane antibody. The resulting data from the prolactin samples supports an exceptional analytical sensitivity, with a calculated Limit of Detection (LoD) of ≤0.625 ng/mL.
A series of seven lateral flow assay (LFA) strips showing the detection of prolactin. The test line is not visible on the first strip, but becomes progressively darker on the remaining strips as the concentration of prolactin increases. The control line remains consistently strong across all concentrations.Prolactin LFA Image
This image of the prolactin Lateral Flow Assay shows that the test line becomes visible at a concentration of 2.5 ng/mL and gets stronger as the concentration increases to 25 ng/mL. The control line remains consistently positive.
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Human Prolactin
Product Concentration
≥ 5.0 mg/ml
Purity
≥95% by SDS Page
Formulation
This purified antibody is supplied in 0.05 M phosphate buffered saline (PBS), pH 7.2 - 7.4, containing 0.1% sodium azide. as a preservative.
State of Matter
Liquid
Storage and Handling
This Purified Antibody is stable when stored at 2-8°C. Do not freeze.
Cross Reactivity
Paired with Leinco Prod. No. P101, Clone No. 174: Pro 100%, BhCG < 1%, ihCG < 1%, LH <1%, FSH < 1%. Paired with Leinco Prod. No. P102, Clone No. 180: Pro 100%, BhCG < 1%, ihCG < 1%, LH <1%, FSH < 1%.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Mouse Anti-Human Prolactin (Clone 172) recognizes Human Prolactin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Prolactin (PRL) or Luteotropic hormone (LTH) is best known as the pituitary modulator of lactation and reproduction.1 Prolactin is a multifaceted hormone that is capable of modulating hundreds of physiological processes in adult vertebrates.2 PRL promotes proliferation, survival and migration of cancer cells acting via the prolactin receptor (PRLR).3 It also modulates maternal behavior and mediates hypothalamic pituitary adrenal axis inhibition during lactation via PRL receptors in the brain.4 Prolactin also has a number of other effects including contributing to surfactant synthesis of the fetal lungs at the end of the pregnancy and immune tolerance of the fetus by the maternal organism during pregnancy. It also decreases normal levels of sex hormones — estrogen in women and testosterone in men.5 Prolactin is a single chain polypeptide of 199 amino acids with a molecular weight of about 24,000 daltons. Its structure is similar to that of growth hormone and placental lactogen. The molecule is folded due to the activity of three disulfide bonds. Significant heterogeneity of the molecule has been described, thus bioassays and immunoassays can give different results due to differing glycosylation, phosphorylation, sulfation, as well as degradation. The non-glycosylated form of prolactin is the dominant form of prolactin that is secreted by the pituitary gland. Increased serum concentrations of prolactin during pregnancy cause enlargement of the mammary glands of the breasts and increases the production of milk. However, the high levels of progesterone during pregnancy act directly on the breasts to stop ejection of milk. It is only when the levels of this hormone fall after childbirth that milk ejection is possible.
PubMed
Research Area
Hormones

References & Citations

1. Paus, R. et al. (2012) Arch Dermatol Res. 304(2):115-8.
2. Zhu, Y. et al. (2008) Comp Biochem Physiol C Toxicol Pharmacol. 148(4):370-80.
3. Panina, S. et al. (2009) J Endocrinol. 201(1):115-28.
4. Neuwmann, ID. et al. (2009) Endocrinology. 150(4):1841-9.
5. Molitch MD., ME. (2005) Mayo Clinic Proceedings 80(8):1050-1057
6. Gout, PW. et al. (1980) Cancer Research 40:2433-36.
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Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.