Purified Recombinant Human SDF-1α (>98%)
<1.0 EU/µg as determined by the LAL method
This antigen affinity purified polyclonal antibody is 0.2 µm filtered and lyophilized from PBS, Ph 7.4, with 5% Trehalose with no carrier proteins or preservatives added.
Storage and Handling
The lyophilized antibody should be stored desiccated at -20 to -70°C. The reconstituted antibody can be stored for at least six weeks at 2 to 8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20 to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: To detect Human SDF-1α this polyclonal antibody can be used at a concentration of 0.1 µg/ml.
Other Applications Reported In Literature ?
Neutralization: Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 1.5-6.0 µg/mL in the presence of 2 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human Stromal Cell-Derived Factor-1Alpha (SDF-1α) recognizes Human SDF-1α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
SDF-1alpha (stromal cell-derived factor-1 alpha) is an alpha-chemokine for haematopoietic stem cells (HSC).1 SDF-1 alpha plays a role in two important processes of granule cell maturation - proliferation and migration - assisting in the achievement of appropriate cell number and position in the cerebellar cortex.2 SDF-1 alpha is also involved in the trafficking of hematopoietic progenitors and stem cells.3 It is expressed by liver cells and in liver diseases.4
NCBI Gene Bank ID
References & Citations
1. Ratajczak, MZ. et al. (2003) Folia Histochem Cytobiol. 41: 13
2. Luster, AD. et al. (2001) Development 128: 1971
3. Vannier, JP. et al. (2002) Eur Cytokine Netw 12: 223
4. Dickenson, J. et al. (2008) Iran J Allergy Asthma Immunol. 7: 61
Products are for research use only. Not for use in diagnostic or therapeutic procedures.