Recombinant Mouse SDF-1α

SDF-1alpha (stromal cell-derived factor-1 alpha) is an alpha-chemokine for haematopoietic stem cells (HSC).¹ SDF-1 alpha plays a role in two important processes of granule cell maturation - proliferation and migration - assisting in the achievement of appropriate cell number and position in the cerebellar cortex.² SDF-1 alpha is also involved in the trafficking of hematopoietic progenitors and stem cells.³ It is expressed by liver cells and in liver diseases.⁴

The biological activity of Mouse SDF-1α was determined by its ability to chemoattract cultured human lymphocytes, and mouse BaF/3 cells transfected with human CXCR4. The expected ED₅₀ for this effect is typically 3-9 ng/ml, and 0.15-0.6 ng/ml, respectively.

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Lys22

Lyophilized

The predicted molecular weight of Recombinant Mouse SDF-1α is Mr 8 kDa.

8

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Ratajczak, MZ. et al. (2003) Folia Histochem Cytobiol. 41: 13
2. Luster, AD. et al. (2001) Development 128: 1971
3. Vannier, JP. et al. (2002) Eur Cytokine Netw 12: 223
4. Dickenson, J. et al. (2008) Iran J Allergy Asthma Immunol. 7: 61