Anti-Human STAT1 (Clone CPTC-STAT1-1) – Purified No Carrier Protein

STAT1 Synthetic peptide YTYEHDPITK

This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

EB0107C-2F2-H2/K2 activity is directed against human STAT1 peptide sequence YTYEHDPITK.

CPTC-STAT1-1

Signal transducer and activator of transcription (STAT) proteins comprise a family of transcription factors involved in various JAK/STAT signaling pathways responsible for immune fitness, inflammation, hematopoiesis, tissue repair, apoptosis, and adipogenesis¹. When STATs are tyrosine-phosphorylated by JAKs, they dimerize and are transported into the nucleus to enact gene regulation, where different STATs have different, nonredundant functions. The STAT family has seven members, STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b, and STAT6, with some members expressing different splice isoforms by cell or tissue type.

STAT1 inhibits cell growth by regulating cell cycle genes and cyclin expression, and also regulates cell differentiation (including granulocytes and osteoblasts), is involved in B cell development, promotes apoptosis, inhibits tumor formation, and regulates the immune system by participating in major histocompatibility complex (MHC)-dependent antigen presentation¹. STAT1 has two isoforms, STAT1α and STAT1β, that are primarily activated by IFN but also by cytokines IL-1 and IL-6 as well as by various growth factors (platelet-derived growth factor, epidermal growth factor, hepatocyte growth factor), tumor necrosis factor, and angiotensin II. Decreased STAT1 expression increases host susceptibility to bacteria, viruses, and parasites.

EB0107C-2F2-H2/K2 was generated for use in immuno-MRM assays in rabbit using a synthetic peptide derived from human STAT1, YTYEHDPITK². EB0107C-2F2-H2/K2 can be used in Western blot analysis to detect STAT1 recombinant protein, but not STAT1 protein from cell lysates³. EB0107C-2F2-H2/K2 does not detect STAT1 by immunohistochemistry or reverse phase protein array.

STAT1 localizes to the cytoplasm and nucleus.

STAT2, IFNAR1, IFNAR2, ERBB4, STAT4, various virus proteins

1 Hu X, Li J, Fu M, et al. Signal Transduct Target Ther. 6(1):402. 2021.
2 Whiteaker JR, Lundeen RA, Zhao L, et al. Front Immunol. 12:765898. 2021.
3 https://antibodies.cancer.gov/detail/SignalTransducerAndActivatorOfTranscription1Peptide1#CPTC-STAT1-1