Anti-Human Thrombopoietin

Pricing & Details

Product No.T432
Clone
34817
Protein
Thrombopoietin
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
THPO, MGC163194, Megakaryocyte Growth and Development Factor (MGDF), MKCSF, ML, MPLLG, MpI Ligand, Leukemia Virus Oncogene Ligand
Isotype
Mouse
IgG2b
Applications
ELISA Cap
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
T432-25 µg
25 µg
$165.00
In stock
Max:
Min: 1
Step: 1
T432-100 µg
100 µg
$420.00
In stock
Max:
Min: 1
Step: 1
T432-500 µg
500 µg
$935.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human TPO (Accession # P40225)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T195 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T155.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human TPO bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human TPO (at 10 ng/ml) is 5-10 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Thrombopoietin (TPO) (Clone 34817) recognizes an epitope on Human TPO. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Thrombopoietin (leukemia virus oncogene ligand, megakaryocyte growth and development factor), also known as THPO is a lineage-dominant hematopoietic cytokine.1 TPO is a glycoprotein hormone constitutively produced mainly by the liver and kidney and is the primary physiological regulator megakaryopoiesis2 and platelet production.1

Antigen Details

References & Citations

1. Vainchenker, W. et al. (1998) Eur Cytokine Newt. 9: 221
2. Skoda, RC. et al. (1998) Blood 92: 4023
Elisa Sandwich Protocol

Formats Available

Prod No.
Description
T432
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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