Anti-Human TSH-ß [Clone 155] — Purified

Anti-Human TSH-ß [Clone 155] — Purified

Product No.: T114

[product_table name="All Top" skus="T114"]

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Clone
155
Target
Thyroid Stimulating Hormone Beta
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
HCG, GPH, GPHA1, CGA, Thyrotropin
Isotype
Mouse IgG

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Data

A line graph showing the dose-response of human TSH, with the average reader values on the y-axis and rhTSH concentration on the x-axis. The curve shows a strong increase in signal as the concentration of rhTSH increases.TSH LOD Graph
The attached graph shows that the gold conjugate (CG) antibody (T114) paired with the nitrocellulose membrane (NCM) antibody (T102) exhibits a strong, broad-range dose-response for human. The data supports an estimated Limit of Detection (LoD) of ≤125 μlU/mL from recombinant TSH (rhTSH) samples.
A series of seven lateral flow assay (LFA) strips showing the detection of recombinant TSH (rhTSH) for T114 and T102. From left to right, the rhTSH concentration increases. The test line is not visible on the first three strips and becomes progressively darker on the remaining strips, while the control line remains consistently strong across all concentrations.TSH LFA Image
The TSH Lateral Flow Assay image shows a clear detection and visible signal increase at the test line, starting at the 500 μlU/mL concentration of rhTSH and strengthening up to 2000 μlU/mL, while the control line remains intensely positive across all concentrations. The match pair used to generate this data was Leinco detection antibody SKU T114 and capture antibody SKU T102.
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human TSH-β (>98%)
Product Concentration
≥ 5.0 mg/ml
Purity
≥95% by SDS Page
Formulation
This purified antibody is supplied in 0.05 M phosphate buffered saline (PBS), pH 7.2 - 7.4, containing 0.1% sodium azide. as a preservative.
State of Matter
Liquid
Storage and Handling
This Purified Antibody is stable when stored at 2-8°C. Do not freeze.
Cross Reactivity
Paired with Leinco Prod. No. T100, Clone No. 154: TSH 100%, BhCG < 1%, ihCG < 1%, LH < 1%, FSH < 1%. Paired with Leinco Prod. No. T103, Clone No. 171: TSH 100%, BhCG < 1%, ihCG ***%, LH < 1%, FSH < 1%. *** inhibition of reaction
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Mouse Anti-Human Thyroid Stimulating Hormone Beta (TSH-β) (Clone 155) recognizes Human Thyroid Stimulating Hormone Beta. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Thyroid-stimulating hormone (TSH) is a glycoprotein hormone1 that regulates the endocrine function of the thyroid gland.2 The most important controller of TSH secretion is thyroid-releasing hormone. Thyroid-releasing hormone is secreted by hypothalamic neurons into hypothalamic-hypophyseal portal blood, finds its receptors on thyrotrophs in the anterior pituitary and stimulates secretion of TSH.1 Independent of age, thyroid cancer incidence correlates with higher TSH. Higher TSH is associated with extrathyroidal extension of disease.3

References & Citations

1. (1998) Bowen A 2. McPherson et al. (2000) Placenta 24: 941 3. Wenger, R H et al. (2003) Wildmann's Clinical Interpretation of Laboratory Tests
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Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.