Anti-Human TNF-β (Clone 5802)

Purified Recombinant Human TNF-β (Accession # P01374)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 0.5-5 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human TNF-β bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human TNF-β (at 0.25 ng/ml) is 0.1-0.2 µg/ml.

Mouse Anti-Human Tumor necrosis factor beta (TNF-β) (Clone 5802) recognizes an epitope on Human TNF-β. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.