Anti-Human Tuberous Sclerosis Complex 1 (CT) (TSC1)

Anti-Human Tuberous Sclerosis Complex 1 (CT) (TSC1)

Product No.: T291

[product_table name="All Top" skus="T291"]

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Target
TSC1
Product Type
Polyclonal Antibody
Alternate Names
Tuberous Sclerosis Complex 1, Hamartin
Applications
WB

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Data

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Rabbit
Product Concentration
0.5 mg/ml
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human Tuberous Sclerosis Complex 1 (TSC1) recognizes an epitope near the C-terminus of Human and Mouse TSC1. This polyclonal antibody was purified using affinity chromatography.
Background
Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1,2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high.3 Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro,4 suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth.4 A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.

Antigen Details

PubMed

References & Citations

1. Shamji, AF. et al. (2003) Mol. Cell 12:271
2. Inoki, K. et al. (2005) Mol. Biol. Rev. 69:79
3. Tabancay Jr., AP. et al. (2003) J. Biol. Chem. 278:39921
4. Inoki, K. et al. (2003) Genes Dev. 17:1829
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.