Anti-Integrin -β3 (Phospho-Tyr773) Antibody (43060)
Data
Immunoblotting: Detection of Integrin β3 (phospho-Tyr773) in extracts
of 293 cells along with the same antibody
preincubated with blocking peptide.
Immunohistochemistry: Detection of Integrin β3 (phospho-Tyr773) in
paraffin-embedded human breast carcinoma tissue. - -
- -
Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of tyrosine 773 (P-L-Y(p)-K-E) derived from human Integrin b3. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~110kDa is detected.
Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to Integrin -beta3 (Phospho-Tyr773) Background The Integrin beta3 (ITGB3) gene encodes glycoprotein IIIa (GP IIIa), the beta subunit of the platelet membrane adhesive protein receptor complex GP IIb/IIIa. The alpha subunit, GP IIb, is encoded by the ITGA2B gene. The GP IIb/IIIa complex belongs to the integrin class of cell adhesion molecule receptors that share a common heterodimeric structure with alpha and beta subunits. Glycoprotein IIIa is also the beta subunit of 2 other integrins, fibronectin receptor and vitronectin receptor which have distinctive alpha subunits. Antigen DetailsResearch Area Phosphospecific Antibodies References & CitationsWB,IHC |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
