Anti-JunB (Phospho-Ser259) Antibody (43027)
Data
Immunohistochemistry: Detection of JunB (phospho-Ser259) in paraffin-embedded human breast carcinoma tissue.
Immunoblot: Detection of JunB (phospho-Ser259) in extracts of
HT29 cells untreated or treated with EGF. - -
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Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of Serine 259 (P-B-S(p)-P-I) derived from human JunB and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~43kDa is detected.
Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to JunB (Phospho-Ser259) Background Transcription factor JunB is a protein that in humans is encoded by the JUNB gene and is involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5′-TGA[CG]TCA-3′. Antigen DetailsFunction Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to an AP-1 consensus sequence and its transcriptional activity (By similarity). {UniProtKB:P09450}. NCBI Gene Bank ID UniProt.org Research Area Phosphospecific Antibodies References & CitationsWB,IHC |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
