Anti-β lactamase Antibody (15720)
Anti-β lactamase Antibody (15720)
Product No.: 15720
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Clone 8A5.A10 Target β-Lactamase Formats AvailableView All Product Type Monoclonal Isotype Mouse IgG1 Applications ELISA , WB |
Data
Nontypeable Haemophilus influenzae (NTHi) isolated from the patient’s throat culture secretes Beta-lactamase-carrying OMVs - -
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Antibody DetailsProduct DetailsReactive Species E. coli Host Species Mouse Immunogen 5'-His-tagged E. coli 205 TEM-1 R+ beta-lactamase. Product Concentration Lot Specific Formulation This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added. State of Matter Liquid Product Preparation Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 10ug/ml. Predicted molecular weight ~29kDa
ELISA: use at 10-20ug/ml (optimized for immobilized beta-lactamase at 10ug/ml). End users should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes TEM-1-type beta-lactamases Background The beta-lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta-lactams are structurally related through the presence of a core beta-lactam ring. Bacterial resistance to beta-lactams continues to increase, primarily due to microbial production of beta-lactamases. Beta-lactamases catalyze the hydrolysis of the betalactam bond which destroys anti-bacterial activity. Bacteria that produce TEM- or SHV-type betalactamases have point mutations in structural genes that have extended the substrate specificity of these beta-lactamases. As a result, many of the beta-lactamase-producing Gram-negative bacteria have become multi-drug resistant. NCBI Gene Bank ID UniProt.org Research Area Enzymes References & CitationsIn Vivo Secretion of β-Lactamase-Carrying Outer Membrane Vesicles as a Mechanism of β-Lactam Therapy Failure. Membranes (Basel) (2021) [34832035] Technical Protocols  Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
