Anti-MAP-2 -Purified

Pricing & Details

Product No.M119
Clone
AP20
Protein
MAP-2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Microtubule Associated Protein 2, MAP2
Isotype
Mouse
IgG1
Applications
IF Staining
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
M119-0.25 mg
0.25 mg
$253.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Bovine brain microtubule associated protein 2
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human MAP-2 (Clone AP20) recognizes the epitope which has been mapped to aa 997-1332 of the MAP-2 protein. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
This antibody is specific to MAP2a and MAP2b, and will not cross-react with MAP1, MAP5, tubulin or tau. This clone reacts with dendrites and cell bodies of neurons but not with the neuronal processes. It does not react with the low MW form (70 kDa, MAP2c).
Background
Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. MAP2 serves to stabilize microtubules (MT) growth by crosslinking MT with intermediate filaments and other MT.

Antigen Details

Protein
PubMed
Research Area
Cell Biology
.
Neuroscience
.
Neuroscience Cell Markers

References & Citations

1. Courtney, MJ. et al. (2013) J Neurosci. 33(19):8185-201. Article Link
2. Iijima, Shinji et al. (2001) J Biochem 129:43-49 PubMed
3. Cacares, A. et al. (1984) J. Neuroscience 4:394
4. Peng, I. et al. (1986) J. Cell Biol. 102:252
5. Lewis, S. A. et al. (1989) Nature 342:498
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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