Anti-Mitosin Antibody (3320)

Product No.: 3320


Clone 14C10 Target Mitosin Formats AvailableView All Product Type Monoclonal Alternate Names CENP-F, AH antigen, Kinetochore protein CENPF, Mitosin Isotype Mouse IgG2a Applications WB,ICC/IF,IHC-P,IHC-Fr,FACS,IP


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Data

CENPF antibody [14C10 1D8] detects CENPF protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. CENPF antibody [14C10 1D8] (3320) dilution: 1:200.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

CENPF antibody [14C10 1D8] detects CENPF protein by western blot analysis. A. 30 ug HeLa whole cell lysate/extract (untreated) B. 30 ug HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr) 5% SDS-PAGE CENPF antibody [14C10 1D8] (3320) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Confocal immunofluorescence staining (Olympus FV10i) of Mitosin. U2OS cells were fixed by 4% PFA and costained with Mitosin 14C10 1D8 AB (Red; cat# 3320; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB, a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen GST fusion protein expressed in E. coli corresponding to aa 1759-2093 of human mitosin. Product Concentration Lot Specific Formulation PBS, pH 7.4 State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-10 ug/mL. Immunoprecipitation: use at 1-10 ug/mL. Immunohistochemistry: use at 10 ug/mL with frozen or paraffin-embedded sections. Positive controls: Human tissue containing rapidly proliferating cells, such as lymphoid germinal centers. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity This antibody recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation. Function Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. {PubMed:12974617, PubMed:17600710, PubMed:7542657, PubMed:7651420}. NCBI Gene Bank ID CENPF UniProt.org P49454 Research Area Cancer Research References & Citations Allred, DC et al. (1997) Lab Invest 15A (69) Zhu, X et al. (1995), Mol Cell Biol 15: 5017-5029. View product citations for antibody PRODUCT_CODE on CiteAb Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

GST fusion protein expressed in E. coli corresponding to aa 1759-2093 of human mitosin.

Product Concentration

Lot Specific

Formulation

PBS, pH 7.4

State of Matter

Liquid

Product Preparation

Purified by Protein G affinity chromatography

Storage and Handling

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.

Regulatory Status

Research Use Only

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

Immunoblotting: use at 1-10 ug/mL.

Immunoprecipitation: use at 1-10 ug/mL.

Immunohistochemistry: use at 10 ug/mL with frozen or paraffin-embedded sections.

Positive controls: Human tissue containing rapidly proliferating cells, such as lymphoid germinal centers.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

This antibody recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.

Function

Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. {PubMed:12974617, PubMed:17600710, PubMed:7542657, PubMed:7651420}.

NCBI Gene Bank ID

CENPF

UniProt.org

P49454

Research Area

Cancer Research