Anti-Human MLH1 Antibody (34077)
Anti-Human MLH1 Antibody (34077)
Product No.: 34077
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Clone G409.1 Target MLH1 Formats AvailableView All Product Type Monoclonal Isotype Mouse IgG2a Applications IHC |
Data
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Recombinant human MLH1 Formulation Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide. State of Matter Liquid Product Preparation Purified by immunoaffinity chromatography Storage and Handling Store at 2-8°C. Do not freeze. Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Monoclonal Antibody specific to human MLH1 Background MutL Homolog 1 (MLH1) is a protein involved in the mismatch-repair pathway. This protein is
commonly associated with hereditary non-polyposis colorectal cancer because the MLH1 gene is
frequently mutated in patients with this cancer. Studies have shown MLH1 to be deficient in a
high percentage of patients with microsatellite instability as well as endometrial and ovarian
cancers. Anti-MLH1 can be useful in the detection of MLH1 in a number of normal and neoplastic
tissues and for identifying a loss of MLH1 in tumors that are microsatellite-unstable Function Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis. {PubMed:16873062, PubMed:18206974, PubMed:20020535, PubMed:21120944, PubMed:9311737}. NCBI Gene Bank ID UniProt.org Research Area Cancer Research References & Citations |
Formats Available
