Anti-Mouse BCA-1 (Clone 143614)

Pricing & Details

Product No.C1424
Clone
143614
Protein
BCA-1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CXCL13, BLC (mouse), BLR1 Ligand, SCYB13, ANGIE, ANGIE2, B Cell-Attracting Chemokine-1
Isotype
Rat
IgG2a
Applications
ELISA Cap
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1424-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
C1424-100 µg
100 µg
$389.00
In stock
Max:
Min: 1
Step: 1
C1424-500 µg
500 µg
$920.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse BCA-1 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:C1502 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:B376.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse BCA-1 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse BCA-1 (at 4 ng/ml) is 12 - 30 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse CXCL13 (Clone 143614) recognizes an epitope on Mouse CXCL13. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23 - 34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

Antigen Details

Protein
PubMed
Elisa Sandwich Protocol

Formats Available

Prod No.
Description
C1424
Products are for research use only. Not for use in diagnostic or therapeutic procedures.