Anti-Mouse CD11α (LFA-1a) [Clone M17/4] — Biotin

C57BL/6 mouse cytotoxic T-cell line T8000

This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

Activity is directed against mouse CD11a, a 180 kDa type I transmembrane glycoprotein also known as the integrin alpha L chain^1,3. Clone M17/4 specifically recognizes an epitope on the extracellular domain of the CD11a subunit¹. This subunit non-covalently associates with the CD18 (integrin beta 2) chain to form the heterodimeric Lymphocyte Function-associated Antigen-1 (LFA-1)^2,3.

LFA-1 (CD11a/CD18) is a critical adhesion molecule belonging to the beta 2 integrin family^3,4. It plays a fundamental role in leukocyte adhesion, trafficking, and the formation of the immunological synapse^5,6.

LFA-1 mediates firm adhesion of leukocytes to the vascular endothelium by binding to its primary ligands: Intercellular Adhesion Molecule-1 (ICAM-1/CD54), ICAM-2 (CD102), and ICAM-3 (CD50)^5,6. This interaction is essential for the extravasation of immune cells into peripheral tissues during inflammatory responses⁵. Additionally, LFA-1 provides crucial co-stimulatory signals during T cell activation by stabilizing the interaction between the T cell receptor (TCR) and the peptide-MHC complex on antigen-presenting cells⁶.

Clone M17/4 is widely recognized for its potent neutralizing and blocking capabilities¹. It is a standard tool in functional bioassays to inhibit LFA-1-mediated cellular adhesion and to study the recruitment of leukocytes in models of infection, autoimmunity, and allograft rejection. Because it targets a monomorphic epitope, M17/4 is highly effective across various mouse strains^1,3.

CD11a is expressed on the surface of all leukocytes, including T and B lymphocytes, monocytes, macrophages, neutrophils, and natural killer (NK) cells^2,3,5. Its expression levels can vary significantly depending on the activation state of the cell^5,6.

ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)

1. Sanchez-Madrid F, et al. Antigens Involved in Mouse Cytolytic T-Lymphocyte (CTL)-Mediated Killing: Functional Screening and Topographic Relationship. Cellular Immunology. 73:1-11. 1982.
2. Davignon D, et al. Monoclonal antibody to a novel lymphocyte function-associated antigen (LFA-1): mechanism of blocking of T lymphocyte-mediated killing and effects on other T and B lymphocyte functions. Journal of Immunology. 127(2):590-595. 1981.
3. Springer TA, et al. LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function. Immunological Reviews. 68:171-195. 1982.
4. Hynes RO. Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 69(1):11-25. 1992.
5. Springer TA. Adhesion receptors of the immune system. Nature. 346(6283):425-434. 1990.
6. Dustin ML, Springer TA. T-cell receptor cross-linking transiently stimulates antigen-specific adherence measured by LFA-1-ICAM-1 interaction. Nature. 341(6243):619-624. 1989.