Anti-Mouse CD11α (LFA-1a) [Clone M17/4] — Purified in vivo PLATINUM™ Functional Grade

C57BL/6 mouse cytotoxic T-cell line T8000

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM ™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

Activity is directed against mouse CD11a, a 180 kDa type I transmembrane glycoprotein also known as the integrin alpha L chain^1,3. Clone M17/4 specifically recognizes an epitope on the extracellular domain of the CD11a subunit¹. This subunit non-covalently associates with the CD18 (integrin beta 2) chain to form the heterodimeric Lymphocyte Function-associated Antigen-1 (LFA-1)^2,3.

LFA-1 (CD11a/CD18) is a critical adhesion molecule belonging to the beta 2 integrin family^3,4. It plays a fundamental role in leukocyte adhesion, trafficking, and the formation of the immunological synapse^5,6.

LFA-1 mediates firm adhesion of leukocytes to the vascular endothelium by binding to its primary ligands: Intercellular Adhesion Molecule-1 (ICAM-1/CD54), ICAM-2 (CD102), and ICAM-3 (CD50)^5,6. This interaction is essential for the extravasation of immune cells into peripheral tissues during inflammatory responses⁵. Additionally, LFA-1 provides crucial co-stimulatory signals during T cell activation by stabilizing the interaction between the T cell receptor (TCR) and the peptide-MHC complex on antigen-presenting cells⁶.

Clone M17/4 is widely recognized for its potent neutralizing and blocking capabilities¹. It is a standard tool in functional bioassays to inhibit LFA-1-mediated cellular adhesion and to study the recruitment of leukocytes in models of infection, autoimmunity, and allograft rejection. Because it targets a monomorphic epitope, M17/4 is highly effective across various mouse strains^1,3.

CD11a is expressed on the surface of all leukocytes, including T and B lymphocytes, monocytes, macrophages, neutrophils, and natural killer (NK) cells^2,3,5. Its expression levels can vary significantly depending on the activation state of the cell^5,6.

ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)

1. Sanchez-Madrid F, et al. Antigens Involved in Mouse Cytolytic T-Lymphocyte (CTL)-Mediated Killing: Functional Screening and Topographic Relationship. Cellular Immunology. 73:1-11. 1982.
2. Davignon D, et al. Monoclonal antibody to a novel lymphocyte function-associated antigen (LFA-1): mechanism of blocking of T lymphocyte-mediated killing and effects on other T and B lymphocyte functions. Journal of Immunology. 127(2):590-595. 1981.
3. Springer TA, et al. LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function. Immunological Reviews. 68:171-195. 1982.
4. Hynes RO. Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 69(1):11-25. 1992.
5. Springer TA. Adhesion receptors of the immune system. Nature. 346(6283):425-434. 1990.
6. Dustin ML, Springer TA. T-cell receptor cross-linking transiently stimulates antigen-specific adherence measured by LFA-1-ICAM-1 interaction. Nature. 341(6243):619-624. 1989.