Anti-Mouse CD11a (Clone I21/7) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD11a (Clone I21/7) – Purified in vivo GOLD™ Functional Grade
Product No.: C376
Clone I21/7 Target CD11a Formats AvailableView All Product Type Monoclonal Antibody Alternate Names LFA-1α Isotype Rat IgG2a Applications FA , FC , in vivo |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Whole T cells Lymphoma BW5147 cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737479 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this I21/7 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone I21/7 recognizes an epitope on mouse CD11a. Background LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis. Antigen Distribution CD11a is present on thymocytes, blood lymphocytes, bone marrow cells and certain lymphoma and macrophage-like cell lines. Ligand/Receptor ICAM-1 (CD54), ICAM-2(CD102), ICAM-3(CD50) Function Intercellular adhesion PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Costimulatory Molecules . Immunology . Signal Transduction Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Common In Vivo Applications of Clone I21/7 in Mice: Clone I21/7 is an anti-mouse CD11a antibody, which targets the LFA-1α subunit. This antibody is used in various in vivo applications related to immune cell studies in mice. The primary applications include:
Overall, clone I21/7 is a valuable tool for immunologists studying immune cell interactions, immune responses, and potential therapeutic interventions in mice. Commonly used antibodies or proteins employed with I21/7 (anti-mouse CD11a, LFA-1α) in the literature include antibodies targeting key leukocyte, T cell, and adhesion markers as well as functional cytokines for combinatorial immune modulation.
Context and rationale:
Technical note: Selection of co-administered antibodies will vary based on application (in vivo depletion/blockade, flow cytometry, immunohistochemistry). When using I21/7 specifically, antibody panels often include isotype controls and may be tailored with additional markers (Gr-1, B220, CD25, NK1.1) for immune profiling in research settings. Summary table of commonly co-used antibodies/proteins with I21/7 (anti-CD11a):
These combinations are broadly supported in immunology and oncology studies focusing on leukocyte trafficking, T cell biology, and immune modulation. Based on the available information, there are no clear or direct key findings from scientific literature regarding clone I21/7 citations. Clone I21/7 is an antibody that recognizes an epitope on mouse CD11a (also known as LFA-1α). This antibody is used as a research tool for studying CD11a, which is part of the LFA-1 integrin complex formed when CD11a combines with CD18. What Clone I21/7 TargetsThe antibody specifically recognizes CD11a, which plays important roles in immune function. LFA-1 (the complex formed by CD11a and CD18) serves as a receptor for several intercellular adhesion molecules including ICAM1, ICAM2, ICAM3, ICAM4, and F11R. This molecule participates in critical immune processes such as the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells, leukocyte-endothelial cell interactions, cytotoxic T-cell mediated killing, and natural killer cell cytotoxicity. The absence of functional LFA-1α or its β chain can lead to leukocyte adhesion deficiency (LAD). However, while the antibody clone I21/7 is commercially available as a research reagent for studying these processes, the search results indicate that specific published findings using this particular clone have not been documented or compiled in readily accessible scientific literature databases. Dosing regimens of clone I21/7 (anti-mouse CD11a, LFA-1α) vary across mouse models primarily in dose amount and frequency, often tailored to the disease model and desired pharmacodynamic effect. In tumor models, daily regimens of 50 µg/dose and low-dose regimens as low as 12 µg/day show similar efficacy, while schedule and route of administration (e.g., subcutaneous or intraperitoneal) also differ by model. Key variations by mouse model:
These regimens reflect efforts to balance efficacy, toxicity, and practical administration, and are based on empirical data as well as mathematical PK/PD modeling in each mouse strain and tumor context. Variations can be significant between models, emphasizing the need for regimen optimization in each context. References & Citations1. Trowbridge, I.S. et al. (1981) J. Exp. Med. 154:1517
2. Springer, T. et al. (1982) Immunol. Rev. 68: 171
3. Wuthrich, R. P. et al. (1991) Cell Immunol. 144:22
3. Falanga, P. B. et al. (1991) Eur. J. Immunol. 21: 2259
4. Talento, A. et al. (1993) Transplantation. 55: 418
5. Barret, T.B. et al. (1991) J. Immunol. 146: 1722 Technical ProtocolsCertificate of Analysis |
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