Anti-Mouse CD11a (Clone FD441.8) – Purified in vivo PLATINUM™ Functional Grade

Clone FD441.8 is an anti-mouse CD11a (LFA-1?) monoclonal antibody that is widely used in in vivo mouse studies for functional modulation of the LFA-1 integrin, particularly in immune system research. Its primary applications are:

• In vivo blockade (neutralization) of LFA-1 function: FD441.8 is frequently used to disrupt the interaction between LFA-1 and its ligands (ICAM-1/2/3) on leukocytes, which interferes with leukocyte adhesion, migration, and related immunological responses.
• Leukocyte trafficking and immune cell migration studies: By blocking LFA-1, researchers assess the role of this integrin in processes such as T cell and leukocyte adhesion to endothelium and migration into tissues during inflammation or immune reactions.
• Functional assays in autoimmunity, infection, transplant, and inflammation models: FD441.8 helps elucidate the importance of lymphocyte function–associated antigen-1 (LFA-1) in various pathophysiological contexts relevant to immune cell-mediated diseases.

Key technical details:

• Isotype: Rat IgG2b.
• Reported use: Neutralization/blockade in vivo, flow cytometry (FC), functional assays (FA), and sometimes in vitro studies.
• Formulation: Typically provided in PBS with ultra-low endotoxin certification for in vivo use.
• Storage: Generally stored at 4°C; do not freeze.

Example applications:

• Injected into mice to temporarily block LFA-1 and assess consequent effects on immune cell distribution, tissue infiltration, or disease outcomes.
• Used alongside isotype controls to confirm specificity of effects.

Summary: FD441.8 is a well-validated tool for transiently inhibiting CD11a (LFA-1) function in vivo in mice, supporting studies of leukocyte biology, immune trafficking, and disease mechanisms involving integrins.

If a specific experimental context or protocol is needed, please specify the area of research or disease model of interest.

The correct storage temperature for sterile packaged clone FD441.8 (HyClone™ FetalClone™ I serum) is ?10?°C or lower for long-term storage. Once thawed, the product should be stored at 2?°C to 8?°C. This temperature range maintains sterility and product quality.

• Long-term storage: Store at ?10?°C or lower.
• After thawing: Store at 2?°C to 8?°C, typically not longer than six weeks if opened.
• If storage is needed beyond six weeks post-opening, aliquot and refreeze.

These recommendations are consistent with manufacturer guidance for HyClone FetalClone I and other similar serum products.

Commonly used antibodies or proteins studied alongside FD441.8 (anti-mouse CD11a/LFA-1?) in the literature focus on cell surface molecules or adhesion complexes that interact with or complement the role of LFA-1 in immune functions.

Key examples include:

• CD18 (integrin ?2): CD11a (recognized by FD441.8) pairs with CD18 to form the functional LFA-1 heterodimer. Experimental setups involving FD441.8 may also include antibodies against CD18 to assess the full LFA-1 complex or compare blocking effects.

• ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50): These are the key ligands for LFA-1. Antibodies or labeled proteins directed against these intercellular adhesion molecules are often used in parallel to study binding, cell adhesion, or blocking interactions involving LFA-1.

• Isotype controls (Rat IgG2b): Experimental controls using the same isotype as FD441.8 (Rat IgG2b, ?) are standard for precise gating and signal validation, such as FITC Rat IgG2b, ? Isotype Control[LTF-2].

• Other leukocyte markers and functional antibodies: Depending on the specific immune cell subset targeted, combinations might include anti-CD3 (T cells), anti-CD4, anti-CD8, anti-CD45 (leukocytes), or cytokine-blocking antibodies. These help to delineate cell populations or parse signaling pathways in conjunction with LFA-1 activity.

In summary, the most commonly paired antibodies with FD441.8 target CD18, ICAM family members, and relevant isotype controls. Additional markers depend on the experimental focus but generally reflect the context in which LFA-1/CD11a function is being explored, such as T-cell activation, leukocyte adhesion, or immune cell migration.

Clone FD441.8 in scientific literature refers to a monoclonal antibody directed against mouse CD11a (LFA-1?), commonly used for immunological research, particularly in blocking studies and cell labeling. The key findings supported by clone FD441.8 citations include:

• CD11a (LFA-1?) Targeting: FD441.8 is a rat-produced monoclonal antibody of isotype IgG2b that recognizes CD11a expressed on mouse thymocytes, blood lymphocytes, bone marrow cells, and certain lymphoma and macrophage-like cell lines.
• Functional Blocking: This clone is frequently utilized in experiments to block LFA-1 function, which is crucial for cell adhesion, migration, and immunological synapse formation between lymphocytes and antigen-presenting cells.
• Applications: Typical research applications for FD441.8 include blocking assays (inhibiting LFA-1-dependent interactions), immunohistochemistry (IHC), immunoprecipitation (IP), and in situ hybridization (ISH).
• Adoptive Immunotherapy Studies: FD441.8 has been instrumental in studies involving antibody-mediated B-cell depletion prior to adoptive immunotherapy. It is often referenced in protocols involving flow cytometry to analyze the effects of LFA-1 blockade on immune cell interactions, transplant rejection, and immune modulation.

No direct findings specific to FD441.8 about clone-initiating cell (CIC) tracking or metastatic studies are reported in the citations provided. Its main impact in the literature is its role as a tool for functional inhibition and cell labeling in mouse immunology.