Anti-Mouse CD11a (Clone FD441.8) – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD11a (Clone FD441.8) – Purified in vivo PLATINUM™ Functional Grade
Product No.: C603
Clone FD441.8 Target CD11a Formats AvailableView All Product Type Monoclonal Antibody Alternate Names LFA-1α Isotype Rat IgG2b Applications FA , FC , N , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Cloned CTL B18 cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829776 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this FD441.8 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a 100 µl volume or 100 µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone FD441.8 recognizes an epitope on mouse CD11a. Background LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis. Antigen Distribution CD11a is present on thymocytes, blood lymphocytes, bone marrow cells and certain lymphoma and macrophage-like cell lines. Ligand/Receptor ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50) Function LFA-1 plays a central role in leukocyte intercellular adhesion. PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Costimulatory Molecules . Immunology . Signal Transduction Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone FD441.8 is a rat monoclonal antibody that targets mouse CD11a (also known as LFA-1α). It is commonly used in in vivo applications in mice for the following purposes:
In summary, Clone FD441.8 is primarily used for LFA-1 neutralization in in vivo studies, facilitating the investigation of immune functions and cell adhesion processes in mice. Commonly Used Antibodies and Proteins with FD441.8 in the LiteratureAnti-CD11a (FD441.8) is a rat monoclonal antibody specific for mouse CD11a (LFA-1α, also known as integrin alpha-L), a key component of the LFA-1 integrin complex involved in leukocyte adhesion and migration. In published research, FD441.8 is frequently used alongside other antibodies and proteins to study immune cell interactions, adhesion, and trafficking. Here are some of the most commonly co-used reagents and their specific roles: Blocking, Functional, and Isotype Controls
Co-Used Monoclonal Antibodies for Costimulation BlockadeIn the context of immune response modulation, especially in transplant and autoimmune research, FD441.8 (anti-mouse CD11a) is often paired with antibodies targeting costimulatory molecules to achieve synergistic immunosuppression:
Antibody Panels for Flow Cytometry and ImmunophenotypingFD441.8 is often included in multi-color flow cytometry panels to identify and characterize immune cell subsets. While the specific combinations vary by experiment, common co-stains might include:
Functional Assays and Cell Migration Studies
Summary Table: Key Antibodies and Proteins Used with FD441.8
Typical Experimental Contexts
ConclusionFD441.8 is most commonly used with antibodies targeting costimulatory molecules (especially CD154), isotype controls, and a range of cell lineage markers for comprehensive immunophenotyping. Its use in blocking studies often involves complementary adhesion molecule blockers to dissect complex leukocyte trafficking pathways. The specific combination depends on the experimental question, but these pairings are well established in the immunology literature. Key Scientific Findings Involving Clone FD441.8FD441.8 is a rat monoclonal antibody (rat IgG2b isotype) specifically recognizing mouse CD11a (LFA-1α), a subunit of the LFA-1 (Lymphocyte Function-Associated Antigen-1) integrin that mediates leukocyte adhesion and immune synapse formation. It is widely used in immunological research for blocking LFA-1 function, flow cytometry, and related applications. Below are the key findings and applications from the scientific literature citing clone FD441.8: Functional Studies
Technical and Preclinical Applications
Summary Table: Major Biological Effects of FD441.8-Mediated LFA-1 Blockade
ConclusionClone FD441.8 is a pivotal tool for dissecting LFA-1 (CD11a) function in murine immunology, with a strong track record in blocking leukocyte adhesion, immune synapse formation, and modulating lymphocyte trafficking. Its specific, functional blockade has provided direct evidence for the critical role of LFA-1 in immune responses, antigen presentation, and transplant rejection, and it remains widely used in both basic and translational research. Dosing Regimens of clone FD441.8 (Anti-LFA-1) in Different Mouse ModelsClone FD441.8 is a monoclonal antibody targeting LFA-1 (CD11a/CD18), commonly used in mouse models to study immune responses, particularly in transplantation, inflammation, and immune cell trafficking research. Experimental Transplant ModelIn a cardiac allograft model, mice (C57BL/6 and A/J strains) received intraperitoneal injections of anti-LFA-1 mAb (clone FD441.8) at 0.2 mg (200 μg) per mouse on days –1 and 0, or days +3 and +4 post-transplant. This schedule was designed to transiently block LFA-1 during critical early phases of alloantigen recognition and graft rejection. Notably, this dosing was used alongside other immune-modulating antibodies and was shown to impact alloreactive T-cell responses and graft outcome. Xenotransplantation/Pancreatic Tissue ModelIn a pig-to-mouse pancreatic tissue transplantation study, dosing of anti-LFA-1 (clone FD441.8, from the same source and presumably the same preparation) was given intraperitoneally (i.p.) at 200 μg/mouse on days 0, 2, 4, and 6 post-transplant, with a single injection repeated biweekly until 3 months post-transplant. This regimen was part of a broader immune suppression protocol and aimed for sustained LFA-1 blockade to promote engraftment and prevent graft rejection. Comparison Table
Key Observations
Practical Considerations
SummaryThe dosing regimen for clone FD441.8 (anti-LFA-1) in mouse models is 200 μg per mouse, intraperitoneally, but the frequency and duration of administration vary according to the experimental model and desired outcome—acute dosing for short-term immune blockade or repeated dosing for sustained suppression. This flexibility allows researchers to tailor the regimen to the specific biological processes under investigation, while the per-dose amount remains consistent across published protocols. References & Citations1. Suratt, Benjamin T. et al. (2009) J Immunol.182(1): 604–612. PubMed 2. Wuthrich, R. P. et al. (1991) Cell Immunol. 144:22 3. Sanchez-Madrid, F. et al. (1983) J. Exp. Med. 158(20):586 4. Pont, S. et al. (1986) J. Immunol. 136(10):3750 Technical ProtocolsCertificate of Analysis |
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