Anti-Mouse CD120b (TNFR2) [Clone TR75-54.7] — Alexa Fluor® 488

E. coli -expressed mouse Type II TNFR

This Alexa Fluor® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Alexa Fluor® is a trademark of Life Technologies Corporation.

Liquid

This Alexa Fluor® 488 conjugate is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2-8°C Wet Ice

Blue Laser (488 nm) Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Armenian Hamster Anti-Mouse Tumor Necrosis Factor Receptor II (TNF RII) (Clone TR75-54.7) recognizes an epitope on Mouse TNF RII. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Tumor necrosis factor receptor II (TNF-RII) or CD120b is a 75kD type I transmembrane protein present on most cell types at low levels¹ including endothelial cells, cardiac myocytes and prostate cells²; the expression is upregulated upon activation. This receptor binds both TNF-α & TNF-β (aka. LT-α). In association with TRAF1 and TRAF2, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state. The TR75-54.7 antibody has been shown to block ligand-induced receptor signaling.

1. Zuckerman, KS. et al. (1998) Cancer Res. 58: 2217
2. Chan, FKM. et al. (2000) Science 288:2351
3. Loetscher, H. et al. (1990) Cell 61:351
4. Rothe, J. et al. (1993) Nature 364:798