Anti-Mouse CD183 (Clone CXCR3-173) – Purified in vivo PLATINUM™ Functional Grade

Home/Bulk In vivo Functional Grade Antibodies, Platinum Purified Bulk Antibodies, Primary Monoclonal Antibodies/Anti-Mouse CD183 (Clone CXCR3-173) – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C796
Clone
CXCR3-173
Protein
CXCR3
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CXCR3G, protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R )
Isotype
Armenian Hamster
IgG
Applications
FC
,
N
Prod No.
Size
Price
Avail.
Qty
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C796-1.0mg
1.0 mg
$225.00
In stock
Max:
Min: 1
Step: 1
C796-5.0mg
5.0 mg
$445.00
In stock
Max:
Min: 1
Step: 1
C796-25mg
25 mg
$1,285.00
In stock
Max:
Min: 1
Step: 1
C796-50mg
50 mg
$2,045.00
In stock
Max:
Min: 1
Step: 1
C796-100mg
100 mg
$2,800.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Armenian Hamster
Immunogen
Mouse N-terminus of CXCR3
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC
Other Applications Reported In Literature ?
N
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
CXCR3-173 activity is directed against murine CD183 (CXCR3).
Antigen Distribution
CXCL3 is expressed on primary memory phenotype CD4+ and CD8+ T cells, naturally occurring CD4+CD25+ Foxp3+ regulatory T cells, natural killer (NK) T cells, and approximately 25% of NK cells. CXCR3-173 recognizes an epitope of CXCR3 expressed on the surface of activated mouse splenocytes.
Background
CXCR3 (CD183) is a chemokine receptor that binds to three ligands, CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (ITAC), that are induced by IFNγ, -α/β, or other proinflammatory cytokines1. CXCR3 is important for natural killer (NK) cell-dependent priming of CD4+ T cells in lymph nodes2, host responses to infection3, and CD4+ T cell responses to allografts4,5.

CXCR3-173 was generated by immunizing Armenian hamsters with a peptide sequence unique to mouse CXCR3 which encompasses amino acids 1-376,7. Hamsters were tested by ELISA for seropositivity against CXCR3 peptide and hybridomas were generated, screened by FACS, purified, and tested for: staining, CXCR3 blockade in vitro, and endotoxin levels6. CXCR3-173 detects the native form of CXCR3, and therefore does not work in Western blotting.

CXCR3-173 has potential use in immunotherapeutic approaches to inhibit transplant rejection and immune related diseases6. In vitro, CXCR3-173 blocks chemotaxis in response to CXCL10 or CXCL11 but not CXCL9. In vivo, CXCR3-173 prolongs both cardiac and islet allograft survival in a manner further enhanced by rapamycin.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Tokunaga R, Zhang W, Naseem M, et al. Cancer Treat Rev. 63:40-47. 2018.
2. Martin-Fontecha A, Thomsen LL, Brett S, et al. Nat Immunol. 5: 1260-1265. 2004.
3. Khan IA, MacLean JA, Lee FS, et al. Immunity. 12: 483-494. 2000.
4. Hancock WW, Lu B, Gao W, et al. J Exp Med. 192: 1515-1520. 2000.
5. Hancock WW, Gao W, Csizmadia V, et al. J Exp Med. 193: 975-980. 2001.
6. Uppaluri R, Sheehan KC, Wang L, et al. Transplantation. 86(1):137-147. 2008.
7. Krug A, Uppaluri R, Facchetti F, et al. J Immunol. 169(11):6079-6083. 2002.
Flow Cytometry

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.