Anti-Mouse CD3 – Purified (PhenoCyler-Fusion (CODEX)® Ready)
Anti-Mouse CD3 – Purified (PhenoCyler-Fusion (CODEX)® Ready)
Product No.: C513
- -
- -
Clone 17A2 Target CD3 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names T-cell Receptor Complex, CD3ε Isotype IgG2b κ Applications IHC FF , PhenoCycler® |
- -
- -
CODEX® DetailsValidation Notes Clone 17A2 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue. Tissue Screened Spleen Tissue Preparation FF (Fresh Frozen) Antibody and Reporter DetailsReactivity Species Mouse Host Species Rat Concentration 0.5 mg/ml Immunogen γ/δ TCR-positive T-T hybridoma D1 Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using <i>in vitro</i> cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Applications and Recommended Usage? Quality Tested by Leinco CODEX® This CD3 (Clone 17A2) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml. RRID AB_2893570 Country of Origin USA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone 17A2 recognizes an epitope on mouse CD3. Antigen Distribution CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells. Background CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition. Antigen DetailsProtein Ligand/Receptor Peptide antigen/MHC-complex Function Antigen recognition, TCR signal transduction, T cell activation PubMed NCBI Gene Bank ID References & Citations1. Gallimore, A. et al. (2005) J Immunol. 175(11):7098-102. PubMed 2. Mysliwietz, J. et al. (1992) Blood 80: 2661-2667 Technical Protocols PhenoCycler® |
Formats Available
- -
- -
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
