Anti-Mouse CD370 (CLEC9A/DNGR1) [Clone 1F6] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD370 (CLEC9A/DNGR1) [Clone 1F6] — Purified in vivo GOLD™ Functional Grade
Product No.: I-2001
Clone 1F6 Target CD370 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD370, DNGR-1, C-Type Lectin Domain Family 9 Member A Isotype Mouse IgG1 Applications FC , IF , in vivo , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen RBL-2H3 cells expressing mouse DNGR-1 fused to an HA epitope Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2893827 Applications and Recommended Usage? Quality Tested by Leinco FC WB Additional Applications Reported In Literature ? IF Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This clone 1F6 monoclonal antibody recognizes a direct epitope on mouse DNGR1/CLEC9A for CD8+ dendritic cells, which can be exploited for tumor therapy, whilst also recognizing necrotic cells. This antibody can recognize both the short and long isoform of DNGR-1, which varies in a segment of the stalk region. Background CLEC9A antibody, 1F6, recognizes C-type lectin domain family 9 member A (CLEC9A), also known as CD370 and DNGR-1. CLEC9A is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily. In mice and humans, CLEC9A is expressed by CD8a+ conventional dendritic cells (cDCs) in mice1,2 and BDCA3+ DCs in humans3, which are specialized for the cross-presentation of exogenous antigens on MHC class I4. In mice, type I interferon (IFN)-secreting plasmacytoid DCs (pDCs) also express CLEC9A2. The ligand for CLEC9A is filamentous actin exposed on apoptotic cells5,6, and ligation of CLEC9A results in the cross-presentation and induction of CD8 T cell responses. CLEC9A is a target for vaccine enhancement, and antibodies targeting CLEC9A in vaccination studies lead to enhanced presentation by DCs, eliciting CD8 and CD4 T cell proliferation and strong humoral immunity Antigen Distribution CD370 (CLEC9A) in mice is expressed on CD8+ DCs and at low levels by plasmacytoid DCs PubMed NCBI Gene Bank ID UniProt.org Q8BRU4 Research Area Immunology . Innate Immunity Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone 1F6 is a monoclonal antibody that serves as a valuable research tool for targeting CD370 (CLEC9A/DNGR1) in in vivo mouse studies, particularly for immunological research and cancer therapy investigations. Target and SpecificityThe 1F6 clone specifically recognizes and binds to mouse CD370 (CLEC9A/DNGR1), which is expressed primarily on CD8+ dendritic cells and at lower levels on plasmacytoid dendritic cells. This antibody is derived from rat and belongs to the IgG1 isotype, making it suitable for in vivo applications. Applications in Mouse StudiesDendritic Cell Research: The primary use of clone 1F6 in in vivo studies is to target and study CD8+ dendritic cells, which are crucial components of the adaptive immune system. Since CD370 is selectively expressed on these cells, the antibody allows researchers to specifically investigate dendritic cell function, distribution, and behavior in living mice. Cancer Therapy Research: Clone 1F6 has particular significance in tumor therapy research, as the CD8+ dendritic cells it targets play important roles in anti-tumor immune responses. Researchers can use this antibody to explore how targeting these cells might enhance immune responses against tumors. Vaccine Research: Given that CLEC9A is being investigated for its potential to enhance vaccine-induced immune responses, clone 1F6 serves as a tool to study how targeting this receptor might improve vaccination outcomes in mouse models. Technical Specifications for In Vivo UseThe 1F6 antibody is specifically formulated for in vivo applications with low endotoxin levels (?1.0 EU/mg), which is critical for preventing unwanted inflammatory responses that could confound experimental results. It's provided in PBS buffer without carrier proteins, potassium, or preservatives, and maintains high purity (>95-98%) suitable for injection into living animals. The antibody is available in various bulk sizes ranging from 1mg to 100mg, with multi-gram quantities available for larger studies, making it practical for different scales of in vivo experiments. Based on the available search results, I cannot find specific storage temperature information for "sterile packaged clone 1F6." The search results contain temperature storage guidelines for various laboratory reagents and pharmaceutical products, but none specifically mention clone 1F6. However, the search results do provide general storage temperature ranges that are commonly used for sterile biological products: Standard Storage Temperature RangesFreezer Storage: -25°C to -10°C (-13°F to 14°F) Refrigerator Storage: +2°C to +8°C (35.6°F to 46.4°F) Cool Storage: +8°C to +15°C Controlled Room Temperature: +20°C to +25°C Common Storage Practices for Similar ProductsThe search results show that related clone detection reagents and media are typically stored at:
RecommendationWithout specific documentation for clone 1F6, refrigerator storage at 2°C to 8°C (35.6°F to 46.4°F) would be the most appropriate choice, as this is the standard storage condition for most sterile biological clones and cell culture products. However, you should consult the product's specific datasheet, certificate of analysis, or contact the manufacturer directly to confirm the exact storage requirements for clone 1F6, as different clones may have varying stability requirements. Based on the available information, the most commonly documented pairing with 1F6 antibodies involves ELISA detection systems and research applications targeting specific proteins. IL-36 alpha Detection SystemThe human IL-36 alpha/IL-1F6 antibody (clone 1F6) is frequently used in conjunction with its rabbit monoclonal counterpart (MAB10783) for ELISA applications. This pairing creates a sandwich ELISA system where:
Supporting Reagents and ProteinsIn ELISA applications using 1F6, several standard laboratory reagents are consistently employed:
Research Context ApplicationsThe CD370-targeting 1F6 clone is used in flow cytometry, immunofluorescence, and western blotting applications targeting mouse CD370 (also known as DNGR-1 or C-Type Lectin Domain Family 9 Member A). While specific protein partners aren't detailed in the available literature, this antibody would typically be used alongside standard research reagents like secondary antibodies, fluorescent conjugates, and detection systems appropriate for each application method. The literature suggests that 1F6 antibodies are primarily utilized as part of established detection systems rather than in complex multi-protein interactions, with their main value lying in their specificity for target antigens in research and diagnostic applications. No search results specifically mention clone "1F6" or its citations in scientific literature. Therefore, I cannot provide key findings about this particular clone or its impact in the literature based on the available sources. If you have more context (e.g., species, research field, or publications related to clone 1F6), please provide it, and I can attempt a more targeted search. Otherwise, based on the current results, no information is available about clone 1F6's scientific citations or findings. References & Citations1. Murphy KM, et al. (2012) Blood. 119(25):6052–62 2. Ahmet F, et al. (2008) Blood. 112(8):3264-3273 3. Brown GD, et al. (2008) J Biol Chem. 283(24):16693-701 3. Bevan MJ, et al. (2000) J Exp Med. vol. 192 (pg. 1685-1696) 4. Feest C, et al. (2012) Immunity. 36(4):635–45 5. Kitsoulis S, et al. (2012) Immunity. 36(4):646–57 6. Huang Y, et al. (2011) PNAS. 108(6):2384–9 7. Chan CEZ, et al. (2017) 2:31 8. Joffre OP, et al. (2010) Eur J Immunol. 40(5):1255–65 10. Lahoud M., et al. (2015) Eur. J. Immunol. 45:854–864 11. Caminschi I., et al. (2011) J Immunol. 187(2):842-50 12. Reis e Sousa C., et al. (2008) J Clin Invest. 118(6):2098-110 Technical Protocols IF   Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
