Anti-Mouse CD370 (CLEC9A/DNGR1) [Clone 1F6] — Purified in vivo GOLD™ Functional Grade

Common In Vivo Applications of Clone 1F6 in Mice

Clone 1F6 is a monoclonal antibody that specifically recognizes mouse DNGR-1 (CLEC9A/CD370), a receptor predominantly expressed on CD8α+ conventional dendritic cells (cDCs) in mice. Below are the key in vivo applications of this antibody in murine research:

Targeting and Characterization of CD8α+ Dendritic Cells

• Identification and Tracking: Clone 1F6 is used to identify, track, and characterize CD8α+ dendritic cells within living mice, leveraging its specificity for CLEC9A, which is largely restricted to this dendritic subset.
• Isolation: The antibody can be employed to isolate CD8α+ cDCs for downstream functional or molecular analyses, given its ability to bind a direct epitope on DNGR-1.
• Functional Studies: Researchers use clone 1F6 to study the role of CD8α+ dendritic cells in immune responses, such as antigen cross-presentation, which is critical for CD8 T cell activation and antitumor immunity.

Tumor Therapy and Immune Modulation

• Therapeutic Targeting: Because CD8α+ dendritic cells are specialized for cross-presenting tumor antigens, clone 1F6 can be exploited to deliver immunomodulatory agents (e.g., toxins, cytokines, or targeting moieties) directly to this subset for tumor therapy.
• Necrotic Cell Recognition: The antibody also recognizes necrotic cells in vivo, which may be relevant for studies of cell death and immunogenic cell death in cancer models.

Mechanistic Insights

• Isoform Detection: Clone 1F6 recognizes both the short and long isoforms of DNGR-1, allowing researchers to investigate potential isoform-specific functions in vivo.
• Plasmacytoid Dendritic Cell Studies: While mainly specific for CD8α+ cDCs, CLEC9A is also expressed by type I interferon-secreting plasmacytoid dendritic cells, enabling broader immune cell characterization.

Summary Table: Key Applications

Conclusion

Clone 1F6 is a versatile tool for in vivo studies focused on CD8α+ dendritic cells in mice, with applications ranging from basic immunology (cell tracking, isolation, functional analysis) to translational research (tumor therapy, immunomodulation). Its specificity for DNGR-1 makes it particularly valuable for dissecting the roles of this dendritic subset in health and disease.

When considering the use of antibodies or proteins alongside "1F6," it appears there might be confusion with different entities referred to as "1F6." However, based on the available information, here are some commonly used antibodies or proteins that might be relevant in contexts where "1F6" is mentioned:

1. IL-36 alpha / IL-1F6 Antibodies: These are used to detect interleukin 36 alpha (IL-36 alpha) in various applications such as Western blot, ELISA, and immunohistochemistry (IHC). They are often used in studies related to inflammation and immune responses.

2. Monoclonal Antibody 1F6 Against NhaA: This antibody targets NhaA, a sodium-proton antiporter, and is used in pH-dependent studies.

3. Neurocan Antibody (1F6): This targets the N-terminal epitope of mouse neurocan and is used in immunofluorescence, immunohistochemistry, immunoprecipitation, and Western blotting.

4. Anti-Mouse CD370 (1F6) Antibody: Used for Flow Cytometry, Immunofluorescence, and Western Blotting in mouse models.

5. Amyloid Beta Antibodies (4G8, 6E10, mOC98, mOC23): These are used in studies related to amyloid beta peptides and are often used to examine the effects of antigen binding across different epitope regions.

These antibodies and proteins are used in diverse biological contexts, and their specific applications depend on the research goal.

I was unable to find specific information about "clone 1F6" in the provided search results. The search results contain general information about animal cloning, molecular cloning techniques, and citation analysis in scientific literature, but none of them reference a specific clone designated as "1F6."

The term "1F6" typically refers to a monoclonal antibody clone designation in immunology and cell biology research. Monoclonal antibody clones like 1F6 are usually developed to target specific antigens or cell markers and are widely used in research applications such as flow cytometry, immunohistochemistry, and Western blotting. However, without access to relevant scientific literature specifically discussing this clone, I cannot provide detailed findings about its characteristics, applications, or scientific significance.

To obtain accurate information about clone 1F6, you would need to search for scientific publications that specifically mention this clone designation, typically in the context of antibody research, cell marker identification, or related immunological studies. The clone designation would usually appear alongside information about what antigen it targets and in which experimental contexts it has been validated.

Based on the provided search results, there is no specific information available about dosing regimens for clone 1F6 across different mouse models. The search results discuss various antibody dosing strategies in mouse models, including anti-PD-1/PD-L1 antibodies, anti-CSF-1R antibodies (clone AFS98), and other therapeutic antibodies, but clone 1F6 is not mentioned in any of these sources.

The search results do indicate that antibody dosing regimens generally vary substantially across mouse models depending on experimental objectives, and that factors such as dose level, dosing interval, and treatment duration can all be adjusted. However, without specific literature on clone 1F6, I cannot provide details about how its dosing regimens vary across different mouse model systems.

To obtain information about clone 1F6 dosing protocols, you would need to consult primary research articles or technical documentation specifically describing experiments using this particular antibody clone.