Anti-Mouse CD370 (CLEC9A/DNGR1) – Purified in vivo GOLD™ Functional Grade
Pricing & Details
RBL-2H3 cells expressing mouse DNGR-1 fused to an HA epitope
≥ 5.0 mg/ml
≤ 1.0 EU/mg as determined by the LAL method
≥95% monomer by analytical SEC
>95% by SDS Page
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Other Applications Reported In Literature ?
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
This clone 1F6 monoclonal antibody recognizes a direct epitope on mouse DNGR1/CLEC9A for CD8+ dendritic cells, which can be exploited for tumor therapy, whilst also recognizing necrotic cells. This antibody can recognize both the short and long isoform of DNGR-1, which varies in a segment of the stalk region.
CD370 (CLEC9A) in mice is expressed on CD8+ DCs and at low levels by plasmacytoid DCs
CLEC9A antibody, 1F6, recognizes C-type lectin domain family 9 member A (CLEC9A), also known as CD370 and DNGR-1. CLEC9A is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily. In mice and humans, CLEC9A is expressed by CD8a+ conventional dendritic cells (cDCs) in mice1,2 and BDCA3+ DCs in humans3, which are specialized for the cross-presentation of exogenous antigens on MHC class I4. In mice, type I interferon (IFN)-secreting plasmacytoid DCs (pDCs) also express CLEC9A2. The ligand for CLEC9A is filamentous actin exposed on apoptotic cells5,6, and ligation of CLEC9A results in the cross-presentation and induction of CD8 T cell responses. CLEC9A is a target for vaccine enhancement, and antibodies targeting CLEC9A in vaccination studies lead to enhanced presentation by DCs, eliciting CD8 and CD4 T cell proliferation and strong humoral immunity
NCBI Gene Bank ID
References & Citations
1. Murphy KM, et al. (2012) Blood. 119(25):6052–62
2. Ahmet F, et al. (2008) Blood. 112(8):3264-3273
3. Brown GD, et al. (2008) J Biol Chem. 283(24):16693-701
3. Bevan MJ, et al. (2000) J Exp Med. vol. 192 (pg. 1685-1696) 4. Feest C, et al. (2012) Immunity. 36(4):635–45
5. Kitsoulis S, et al. (2012) Immunity. 36(4):646–57
6. Huang Y, et al. (2011) PNAS. 108(6):2384–9
7. Chan CEZ, et al. (2017) 2:31
8. Joffre OP, et al. (2010) Eur J Immunol. 40(5):1255–65
10. Lahoud M., et al. (2015) Eur. J. Immunol. 45:854–864
11. Caminschi I., et al. (2011) J Immunol. 187(2):842-50
12. Reis e Sousa C., et al. (2008) J Clin Invest. 118(6):2098-110