Anti-Mouse CD38 [Clone 90] – Purified No Carrier Protein

Mouse bone marrow pre-B cells

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Purified antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Anti-Mouse CD38

CD38 is a 45kDa glycoprotein that can be found both on the cell surface and on the surface of organelles like the endoplasmic reticulum or mitochondria (1). CD38 acts as an enzyme, having both NAD-depleting and intracellular signaling functions, in addition to promoting adhesion when expressed on the cell surface (1). In both mice and humans, CD38 is typically found on immune cells such as B cells, T cells, and NK cells, though in mice it is downregulated in certain types of B cells (2). On immune cells, CD38 regulates critical functions related to inflammation, including cell recruitment/differentiation (3) and cytokine release (4). Because of these inflammatory functions, the role of CD38 in autoimmune diseases is an ongoing area of research (1).

The hybridoma Clone 90 was developed by immunizing rats with mouse bone marrow pre-B cells (5) and has been used in IHC, IP, FC, and blocking studies (5,6).

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1. Piedra-Quintero ZL, Wilson Z, Nava P and Guerau-de-Arellano M (2020) CD38: An Immunomodulatory Molecule in Inflammation and Autoimmunity. Front. Immunol. 11:597959.

2. Shay, T., Jojic, V., Zuk, O., Rothamel, K., Puyraimond-Zemmour, D., Feng, T., ... & ImmGen Consortium. (2013). Conservation and divergence in the transcriptional programs of the human and mouse immune systems. Proceedings of the National Academy of Sciences, 110(8), 2946-2951.

3. Frasca, L., Fedele, G., Deaglio, S., Capuano, C., Palazzo, R., Vaisitti, T., ... & Ausiello, C. M. (2006). CD38 orchestrates migration, survival, and Th1 immune response of human mature dendritic cells. Blood, 107(6), 2392-2399.

4. Sandoval-Montes, C., & Santos-Argumedo, L. (2005). CD38 is expressed selectively during the activation of a subset of mature T cells with reduced proliferation but improved potential to produce cytokines. Journal of leukocyte biology, 77(4), 513-521.

5. Oliver, A. M., Martin, F., & Kearney, J. F. (1997). Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells. Journal of immunology (Baltimore, Md. : 1950), 158(3), 1108–1115.

6. Etich, J., Bergmeier, V., Frie, C., Kreft, S., Bengestrate, L., Eming, S., Mauch, C., Eckes, B., Ulus, H., Lund, F. E., Rappl, G., Abken, H., Paulsson, M., & Brachvogel, B. (2013). PECAM1(+)/Sca1(+)/CD38(+) vascular cells transform into myofibroblast-like cells in skin wound repair. PloS one, 8(1), e53262.