Anti-Mouse CD62L – DyLight® 488

Pricing & Details

Product No.C2095
Clone
MEL-14
Protein
CD62L
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
L-Selectin, Ly-22,LAM-1
Isotype
Rat
IgG2a
Applications
FA
,
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2095-50 µg
50 µg
$85.00
In stock
Max:
Min: 1
Step: 1
C2095-100 µg
100 µg
$150.00
In stock
Max:
Min: 1
Step: 1
C2095-200 µg
200 µg
$250.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
38C-13 cells
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Blue Laser (493 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this MEL-14 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Reported Applications For Relevant Conjugates ?
IP
B
IHC (Frozen)
IHC (Paraffin)
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse CD62L (Clone MEL-14) recognizes an epitope on Mouse CD62L. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody was then conjugated to DyLight 488 under optimal conditions.
Background
CD62L is a 75-95 kD glycoprotein which is also known as L-selectin, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells.

Antigen Details

Protein
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
PubMed
NCBI Gene Bank ID
Research Area
Cell Adhesion
.
Cell Biology
.
Costimulatory Molecules
.
Immunology
.
Innate Immunity
Flow Cytometry

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.