Anti-Mouse CD62L – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.C2118
Clone
MEL-14
Protein
CD62L
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Isotype
Rat
IgG2a κ
Applications
B
,
CyTOF®
,
FA
,
FC
,
IHC FF
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2118-1.0 mg
1.0 mg
$98.00
In stock
Max:
Min: 1
Step: 1
C2118-5.0 mg
5.0 mg
$325.00
In stock
Max:
Min: 1
Step: 1
C2118-25 mg
25 mg
$875.00
In stock
Max:
Min: 1
Step: 1
C2118-50 mg
50 mg
$1,450.00
In stock
Max:
Min: 1
Step: 1
C2118-100 mg
100 mg
$2,125.00
In stock
Max:
Min: 1
Step: 1

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this MEL-14 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this MEL-14 antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
CyTOF®
Additional Reported Applications For Relevant Conjugates ?
IHC (Paraffin)
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse CD62L (Clone MEL-14) recognizes an epitope on Mouse CD62L. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
Subsets of B and T cells, monocytes, granulocytes, subset of NK cells
Background
CD62L is a 74-95 kD glycoprotein is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. Furthermore, CD62L is a cell adhesion molecule that binds to many glycoprotein ligands including CD34, GlyCAM-1, and PSGL-1. Clone MEL-14 is reported as a Nutralization antibody.

Antigen Details

Protein
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Function
Lymphocyte homing to HEV, rolling on activated endothelium
PubMed
NCBI Gene Bank ID
Research Area
Cell Adhesion
.
Cell Biology
.
Costimulatory Molecules
.
Immunology
.
Innate Immunity

References & Citations

1.) Gubin, M. et al. (2018) Cell. 175(4):1014–1030.e19 Journal Link
Flow Cytometry
IHC
Immunoprecipitation Protocol
General Western Blot Protocol