Anti-Mouse CD70 (Clone TAN1-7) – Purified in vivo GOLD™ Functional Grade

Clone TAN1-7 is a monoclonal antibody used in in vivo mouse studies to target and block the interaction between mouse CD70 and its receptor CD27, which plays a key role in B and T cell activation. The antibody specifically binds to a direct epitope on the CD70 molecule, thereby inhibiting costimulatory signals important for immune cell activation.

Key uses of TAN1-7 in in vivo mouse studies include:

• Blocking CD70-CD27 interactions to modulate immune responses, such as limiting T and B cell activation and investigating the functional consequences in models of autoimmunity, transplantation, tumor immunology, and infectious disease.
• Evaluating immune modulation by inhibiting CD70, researchers can study the effects on cytotoxic T lymphocyte function, antibody responses, and the survival of immune or tumor cells in various disease models.
• Applied applications: The antibody is administered to mice in experimental settings, where its quality and lack of contaminants (such as endotoxin) are critical for reliable immune experiments.

TAN1-7 is provided in a format optimized for in vivo use—formulated without preservatives, low in endotoxin, and tested for sterility to avoid experimental artifacts. It is available in various bulk quantities suitable for repeated or large-scale animal studies.

Each research group is advised to optimize dosing and scheduling based on the specific experimental goals, as protocol details (e.g., dosage, route of administration) may vary by application and mouse model.

The correct storage temperature for sterile packaged clone TAN1-7 (Anti-Mouse CD70, clone TAN1-7) is 2–8°C (typical refrigerator temperature). For storage longer than one month, it should be aseptically aliquoted and frozen for best preservation.

• For up to one month: Store sterile as received at 2–8°C.
• For long-term storage: Aseptically aliquot and freeze, typically at –20°C or below (source does not state the exact long-term temperature, but this is standard practice for antibodies unless otherwise specified).

Always consult the specific manufacturer's datasheet for confirmation, as protocols may vary by product batch and format.

In the literature, antibodies or protein markers commonly used with TAN1 (TANGLED1) include AIR9 and POK1; these proteins are frequently studied together due to their roles in division site localization and cytoskeletal organization in plant cells. Specifically, AIR9 is another microtubule-associated protein required for proper POK1 localization, and studies have shown that TAN1 and AIR9 mediate the recruitment of POK1 (PHRAGMOPLAST ORIENTING KINESIN1) to the cell division site.

Other proteins and markers sometimes used in division site or microtubule studies, although not always directly cited with TAN1-7, include:

• Tubulin: As a standard marker for microtubules in plant cells.
• Actin: For cytoskeletal localization studies.
• Division plane markers: Such as KNOLLE or other phragmoplast-localized proteins, when assessing cell division orientation (not explicitly found in the cited paper but commonly used in related studies).

Key insights from recent literature:

• TAN1 and AIR9 antibodies/proteins are used together to study protein-protein interactions responsible for division-site localization and phragmoplast guidance.
• POK1 protein is used to assess localization defects, especially in mutant backgrounds for TAN1 and AIR9, suggesting co-use of antibodies against these proteins in immunolocalization or co-immunoprecipitation assays.

If your interest lies in a specific tissue, developmental stage, or species, the antibody panel may vary, but AIR9 and POK1 are the most frequently referenced in direct interaction studies with TAN1. Literature on structural protein-antigen complexes may also include common immunological markers like bovine serum albumin (BSA), but this is in the context of immunological assays, not cytoskeletal biology.

Based on the available scientific literature, I cannot find specific information about a clone designated as "TAN1-7." However, the search results reveal significant findings about the TAN1 gene itself across multiple research contexts, which may be relevant to understanding TAN1-related research.

TAN1 in Yeast tRNA Modification

The TAN1 gene in Saccharomyces cerevisiae (baker's yeast) has been identified as a crucial component in tRNA modification pathways. The gene product is required for the formation of N4-acetylcytidine (ac4C), a modified nucleoside found in transfer RNA. Research demonstrates that TAN1-null mutants lack this specific compound, which can be restored by complementation with a plasmid carrying the TAN1 gene.

Molecular Characteristics: The Tan1 protein contains a THUMP domain, which is a predicted RNA-binding motif commonly found in enzymes involved in RNA modification, including 4-thiouridine and pseudouridine synthases, as well as RNA methyltransferases. Experimental evidence using gel mobility shift assays has confirmed that the Tan1 protein can bind to tRNA molecules.

TAN1 in Sorghum Tannin Production

In agricultural research, the Tannin1 (Tan1) gene in sorghum has been cloned and characterized as a key regulator of grain tannin production. This gene encodes a WD40 protein, and naturally occurring allelic variants in highly conserved regions cause frame shifts and premature stop codons, resulting in truncated amino acid sequences and the absence of tannins in sorghum grains.

Agricultural Significance: The identification of Tan1 in sorghum has important implications for crop breeding, as tannins affect both nutritional value and palatability of grains. Compared to wild relatives, cultivated sorghum varieties show reduced nucleotide diversity in the Tan1 coding region, suggesting selective pressure during domestication.

Research Methodology and Validation

The cloning of Tan1 genes has employed sophisticated approaches including genetic linkage mapping, fine-mapping through meta-quantitative trait locus analysis, association validation with diverse genetic accessions, and transgenic complementation studies. These comprehensive methodologies have provided robust validation of gene function across different biological systems.

Without access to specific literature citing "TAN1-7" as a distinct clone, these findings represent the current understanding of TAN1 gene function in major research contexts. If you're looking for information about a specific TAN1-7 clone, additional search terms or database queries might be necessary to locate the relevant citations.