Anti-Mouse CD8 [Clone YTS-169] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD8 [Clone YTS-169] — Purified in vivo GOLD™ Functional Grade
Product No.: C2442
Clone YTS-169 Target CD8 Formats AvailableView All Product Type Monoclonal Antibody Isotype Rat IgG2b κ Applications Depletion , FC , IHC FF , in vivo , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen CBA mouse thymocytes Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829540 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this YTS-169 antibody for staining cells in flow cytometry is ≤ 0.2 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this YTS-169 antibody for use in western blotting is 1-10 μg/ml. Additional Applications Reported In Literature ? IHC (Frozen) Depletion Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone YTS-169 recognizes mouse CD8. Background CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes. Antigen Distribution CD8 is expressed on blood lymphocytes, a subset of NK cells, and thymocytes. Persons with HIV exhibit increased levels of CD8+ lymphocytes.
PubMed Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. The YTS-169 clone is extensively used in in vivo mouse studies as a depleting antibody to eliminate CD8+ T cells, making it a valuable research tool for studying immune function and disease models. Primary Applications in Mouse StudiesCD8+ T Cell Depletion: YTS-169 exhibits depleting activity when administered in vivo, effectively removing CD8+ T lymphocytes from the mouse immune system. This depletion capability makes it particularly useful for researchers investigating the role of CD8+ T cells in various biological processes. Cancer Research: The antibody has been utilized in tumor studies where CD8+ T cell depletion is combined with other therapeutic agents. Research has shown that this combination approach can demonstrate greater efficacy than single agents and strongly suppress tumor growth in vivo. Autoimmune Disease Research: YTS-169 has potential applications in the treatment and study of autoimmune diseases, where modulating CD8+ T cell populations can provide insights into disease mechanisms. Technical Specifications for In Vivo UseFormulation: The antibody is available in specialized low-endotoxin formulations specifically designed for in vivo applications. It is supplied in phosphate-buffered saline (PBS) at pH 7.2 with 150 mM NaCl, without carrier proteins, potassium, or preservatives. Purity and Quality: The antibody maintains greater than 95% purity as determined by SDS-PAGE and HPLC, ensuring consistent results in experimental studies. Strain Compatibility and Binding CharacteristicsA key advantage of YTS-169 over other anti-CD8 antibodies is its universal binding capability. Unlike the 2.43 antibody clone which only binds CD8? in Lyt2.2+ mouse strains, YTS-169 binds CD8? in all mouse strains. This broad compatibility makes it the preferred choice for studies involving different mouse genetic backgrounds. The antibody recognizes the murine CD8 cell surface antigen expressed by a subset of T lymphocytes and acts as an antigen co-receptor that mediates efficient cell-to-cell interactions within the immune system. YTS-169 is classified as a rat IgG2b monoclonal antibody that was originally generated using CBA mouse thymocytes as the immunogen. Based on the literature, YTS-169 antibody is commonly used alongside several other antibodies and proteins in research studies, particularly in immunological and vaccine research contexts. Companion AntibodiesThe 2.43 antibody appears most frequently with YTS-169 in comparative studies. Both antibodies bind to mouse CD8? (Lyt2), but they have different specificities - YTS-169 binds both Lyt2.1 and Lyt2.2 epitopes, while 2.43 is specific only for Lyt2.2. These antibodies are often used together in flow cytometry analyses and engineered antibody fragment studies, with both showing similar binding affinities (equilibrium constants of 33 nM for YTS-169 and 34 nM for 2.43). Anti-CD4 antibodies are routinely used in combination with YTS-169 for T cell subset identification. Studies commonly employ phycoerythrin (PE)-conjugated anti-CD4 antibodies alongside FITC-conjugated YTS-169 or other CD8 antibodies to distinguish CD4+ and CD8+ T cell populations. Related CD8-Targeting ProteinsThe YTS156 antibody represents another member of this antibody family, though it has different specificity, targeting the Lyt3 epitope rather than Lyt2. This antibody is often included in comparative binding studies to demonstrate the specificity of YTS-169 and 2.43. Recombinant soluble CD8?? (sCD8??) heterodimer fusion proteins are frequently used as target antigens in studies involving YTS-169. These engineered proteins, created by removing transmembrane domains and fusing CD8? and CD8? with ?-helical linkers, serve as purified antigens for binding affinity measurements and validation studies. Vaccine Research ContextIn malaria vaccine research, YTS-169 appears alongside chimeric recombinant proteins such as PyLPC (P. yoelii recombinant linear peptide chimera) and PyRMC (P. yoelii recombinant modular chimera). These proteins incorporate multiple T cell epitopes and are used in multistage vaccine approaches where immune response monitoring requires CD8+ T cell identification using antibodies like YTS-169. The literature demonstrates that YTS-169 is most commonly paired with other CD8-specific antibodies for comparative studies, anti-CD4 antibodies for T cell subset analysis, and various recombinant proteins in vaccine and immunological research applications. Clone YTS-169 is a widely cited rat monoclonal antibody targeting murine CD8?, and it has been fundamental in immunology for detecting, imaging, and depleting CD8+ T cells in mice. Key findings from scientific literature referencing YTS-169 include:
In summary, clone YTS-169 is valued for its broad strain applicability, reliable CD8+ T cell targeting in both imaging and depletion experiments, and its role in the development of next-generation immuno-imaging agents. These properties make YTS-169 a reference standard in mouse CD8 immunology. Dosing regimens for clone YTS-169 in mouse models vary depending on experimental goals, mouse strain, disease model, and desired degree and duration of CD8+ T cell depletion, but standardized dosing guidance is rarely published directly by vendors or in product datasheets. In contrast, other CD8 depleting clones such as 2.43 have more widely available regimen details for comparison. Key details on dosing of YTS-169 from available sources:
Comparative reference:
Considerations for different mouse models:
Summary Table: YTS-169 vs. 2.43 Dosing Regimens
Summary: The dosing regimen for clone YTS-169 in mouse models is typically 200–400?µg per mouse every 3–7 days by i.p. or i.v. injection, closely mirroring standard protocols for other anti-CD8 clones like 2.43, with adjustments according to strain, experimental goals, and duration of depletion required. It is recommended to consult published studies specific to the disease or model in question for optimal dosing, as direct vendor guidance is limited. References & Citations1. Parnes, J. R. et al. (1989) Adv. Immunol. 44:265 2. Reinherz, E. L. et al. (1980) J. Immunol. 124:1301 3. Fischer, A. et al. (1983) Immunology 48:177 4. Merkenschlanger, M. et al. (1988) Eur. J. Immunol. 18:1653 5. Leukocyte Typing: 3rd Workshop: Code No. 567; 4th Workshop: Code No. N31 Technical ProtocolsCertificate of Analysis |
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