Anti-Human CD8 – Purified In vivo GOLD™ Functional Grade

Pricing & Details

  
Clone
UCHT-4
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Mouse
IgG2a
Applications
FA
,
FC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C366-1.0 mg
1.0 mg
$95.00
In stock
C366-5.0 mg
5.0 mg
$298.00
In stock
C366-25 mg
25 mg
$775.00
In stock
C366-50 mg
50 mg
$1,275.00
In stock
C366-100 mg
100 mg
$1,998.00
In stock
  

Antibody Details

Reactivity Species
Human
Host Species
Mouse
Product Concentration
≥ 2.0 mg/ml
1.0 - 5.0 mg/ml
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
FC The suggested concentration for this UCHT-4 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this UCHT-4 antibody for use in western blotting is 1-10 μg/ml.

Description

Specificity
Clone UCHT-4 recognizes human CD8.
Antigen Distribution
CD8 is expressed on blood lymphocytes, a subset of NK cells, and thymocytes. Persons with HIV exhibit increased levels of CD8+ lymphocytes.
Background
CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes.

Antigen Details

Protein
CD8
Ligand/Receptor
MHC Class I molecules
PubMed
CD8+AND+Human
NCBI Gene Bank ID

References & Citations

1. Parnes, J. R. et al. (1989) Adv. Immunol. 44:265 2. Reinherz, E. L. et al. (1980) J. Immunol. 124:1301 3. Fischer, A. et al. (1983) Immunology 48:177 4. Merkenschlanger, M. et al. (1988) Eur. J. Immunol. 18:1653 5. Leukocyte Typing: 3rd Workshop: Code No. 567; 4th Workshop: Code No. N31
Flow Cytometry
General Western Blot Protocol
  

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.