Anti-Human CD8 – Purified

Pricing & Details

  
Clone
UCHT-4
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
T8, Leu2
Isotype
Mouse
IgG2a
Applications
FC
,
IHC FF
Prod No.
Size
Price
Avail.
Qty
Add to cart
C117-50 µg
50 µg
$50.00
In stock
C117-100 µg
100 µg
$85.00
In stock
C117-200 µg
200 µg
$150.00
In stock
  

Antibody Details

Reactivity Species
Human
Host Species
Mouse
Product Concentration
0.1 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable when stored at 2-8°C. Do not freeze.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
FC The suggested concentration for this UCHT-4 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD8. A suggested method would be to stain cells expressing CD8 with 0.5 µg per million cells in a total staining volume of 100 µl followed by PN:M1189.
Other Applications Reported In Literature ?
IHC (Frozen)
Each investigator should determine their own optimal working dilution for specific applications.

Description

Specificity
Clone UCHT-4 recognizes human CD8.
Antigen Distribution
CD8 is expressed on blood lymphocytes, a subset of NK cells, and thymocytes. Persons with HIV exhibit increased levels of CD8+ lymphocytes.
Background
CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes.

Antigen Details

Protein
CD8
Ligand/Receptor
MHC Class I molecules
PubMed
CD8+AND+Human
NCBI Gene Bank ID

References & Citations

1. Parnes, J. R. et al. (1989) Adv. Immunol. 44:265 2. Reinherz, E. L. et al. (1980) J. Immunol. 124:1301 3. Fischer, A. et al. (1983) Immunology 48:177 4. Merkenschlanger, M. et al. (1988) Eur. J. Immunol. 18:1653 5. Leukocyte Typing: 3rd Workshop: Code No. 567; 4th Workshop: Code No. N31
Flow Cytometry
IHC
  

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.