Anti-Mouse CD8b.2 [Clone 53-5.8] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD8b.2 [Clone 53-5.8] — Purified in vivo GOLD™ Functional Grade
Product No.: C2832
Clone 53-5.8 Target CD8b.2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Lyt-3.2, Ly-3.2 Isotype Rat IgG1 κ Applications Depletion , FA , FC , ICC , in vivo , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Mouse thymus or spleen Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737471 Applications and Recommended Usage? Quality Tested by Leinco WB FC The suggested concentration for this 53-5.8 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? ICC Depletion FA Additional Reported Applications For Relevant Conjugates ? IF staining IHC (Frozen) IP For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 53-5.8 recognizes an epitope on mouse CD8b.2. Background CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes. Antigen Distribution CD8b.2 is expressed on the majority of thymocytes and cytotoxic T cell subsets. Ligand/Receptor MHC class I NCBI Gene Bank ID UniProt.org Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. In Vivo Applications of Clone 53-5.8 in MiceThe monoclonal antibody clone 53-5.8, specific for mouse CD8β (Lyt 3.2), is a widely used reagent in immunological research for in vivo applications using mice. Its primary function is to target and deplete CD8+ T cells, which are crucial for understanding immune responses, disease mechanisms, and therapeutic interventions. Key In Vivo Uses
Typical Protocols
Limitations and Caveats
Summary Table
Clone 53-5.8 is a cornerstone reagent for in vivo CD8+ T cell depletion in mice, enabling precise dissection of the roles these cells play in health and disease. Its use requires careful experimental design and interpretation due to potential residual cell activity and technical detection challenges. The 53-5.8 antibody, which targets mouse CD8b.2, is commonly used in conjunction with other antibodies or proteins for various applications, particularly in immunological studies. Here are some of the other commonly used antibodies or proteins:
These combinations allow researchers to study T cell subsets, their roles in immune responses, and the effects of CD8+ T cell depletion in experimental models. Clone 53-5.8 is a monoclonal antibody targeting mouse CD8β and is widely cited in scientific literature as a reliable and potent tool for CD8+ T cell depletion in vivo. Key findings from its use include:
In summary, clone 53-5.8 is highly regarded for efficient and specific CD8+ T cell depletion, and its use has provided crucial insights into the roles of these cells in infection, immunity, and disease models. Dosing regimens for clone 53-5.8, an anti-mouse CD8β monoclonal antibody, can vary depending on the specific mouse model and experimental design. Here are some general insights into how these regimens might differ across different models:
Overall, while the basic dosing regimen for clone 53-5.8 tends to involve intraperitoneal injections of 5 mg/kg or similar dosages, the specific details can vary based on experimental objectives and the mouse model used. References & CitationsTechnical ProtocolsCertificate of Analysis |
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