Anti-Mouse CD8b.2 [Clone 53-5.8] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD8b.2 [Clone 53-5.8] — Purified in vivo GOLD™ Functional Grade
Product No.: C2832
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Clone 53-5.8 Target CD8b.2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Lyt-3.2, Ly-3.2 Isotype Rat IgG1 κ Applications Depletion , FA , FC , ICC , in vivo , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Mouse thymus or spleen Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737471 Applications and Recommended Usage? Quality Tested by Leinco WB FC The suggested concentration for this 53-5.8 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? ICC Depletion FA Additional Reported Applications For Relevant Conjugates ? IF staining IHC (Frozen) IP For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 53-5.8 recognizes an epitope on mouse CD8b.2. Background CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes. Antigen Distribution CD8b.2 is expressed on the majority of thymocytes and cytotoxic T cell subsets. Ligand/Receptor MHC class I NCBI Gene Bank ID UniProt.org Research Area Immunology References & CitationsTechnical ProtocolsDepletion FA  ICC   Certificate of Analysis |
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Formats Available
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
