Anti-Mouse CD8b.2 [Clone 53-5.8] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD8b.2 [Clone 53-5.8] — Purified in vivo GOLD™ Functional Grade
Product No.: C2832
Clone 53-5.8 Target CD8b.2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Lyt-3.2, Ly-3.2 Isotype Rat IgG1 κ Applications Depletion , FA , FC , ICC , in vivo , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Mouse thymus or spleen Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737471 Applications and Recommended Usage? Quality Tested by Leinco WB FC The suggested concentration for this 53-5.8 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? ICC Depletion FA Additional Reported Applications For Relevant Conjugates ? IF staining IHC (Frozen) IP For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 53-5.8 recognizes an epitope on mouse CD8b.2. Background CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes. Antigen Distribution CD8b.2 is expressed on the majority of thymocytes and cytotoxic T cell subsets. Ligand/Receptor MHC class I NCBI Gene Bank ID UniProt.org Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. The clone 53-5.8 is an anti-mouse CD8? (Lyt 3.2) monoclonal antibody that serves as a powerful tool for CD8+ T cell depletion in in vivo mouse studies. This rat IgG1 antibody specifically targets the ? chain of the CD8 differentiation antigen on mouse cells. Primary Applications in In Vivo StudiesCD8+ T Cell DepletionThe 53-5.8 antibody is primarily used to deplete CD8+ T cells in living mice for experimental purposes. When administered in vivo, this antibody has been shown to completely deplete CD8+ T cells while notably preserving other cell populations. Importantly, it does not deplete CD8+ CD11c+ dendritic cells, making it a selective tool for targeting specific immune cell subsets. Immunological ResearchResearchers utilize this antibody in studies investigating the role of conventional CD8??+ T cells in various disease models and immune responses. The depletion approach allows scientists to examine what happens when CD8+ T cells are removed from the immune system, helping to understand their contribution to immune responses, tumor immunity, and autoimmune conditions. Mechanism and SpecificityThe 53-5.8 clone recognizes an epitope on mouse CD8b.2 and is specific for Ly-3.2 phenotype, showing only low reactivity with Ly-3.1 strains such as AKR, C58, MRL, and PL mice. This specificity is important because CD8 can exist as either a heterodimer (CD8??) or homodimer (CD8??), and this antibody specifically targets the ? chain component. Commercial Availability and FormatsThe 53-5.8 clone is widely available from multiple suppliers in various formats optimized for in vivo use, including low endotoxin formulations specifically designed for animal studies. It's available in bulk quantities ranging from milligrams to multi-gram amounts, reflecting its common use in research. The antibody is also available in different conjugated forms for flow cytometry applications to monitor depletion efficiency. This antibody has become a standard tool in immunological research, with over 100 product citations, demonstrating its widespread acceptance and reliability in the scientific community. The recommended storage temperature for sterile packaged clone 53-5.8 (anti-mouse CD8b.2 antibody) is 2–8°C for short-term storage, typically up to one month. For longer-term storage, aliquoting and freezing at lower temperatures (such as -20°C or -80°C) may be advised, depending on specific product guidance. Key details:
For maximum stability and shelf life, always follow manufacturer-specific recommendations on the datasheet supplied with the antibody batch. Commonly Used Antibodies and Proteins with 53-5.8The 53-5.8 monoclonal antibody specifically targets the mouse CD8? chain (also known as Ly-3.2). Researchers often combine 53-5.8 with other antibodies and proteins to study CD8+ T cell biology, immune depletion, and cellular interactions. Here are some of the most commonly used reagents co-employed with 53-5.8 in the literature: Anti-CD8? Antibodies
Additional T Cell Markers
Depletion and Functional Studies
Functional Assays and Recombinant Proteins
Isotype Controls
Typical Experimental CombinationsA standard experimental setup might involve:
Summary Table
Key Literature Context
These combinations reflect the standard toolkit for studying CD8+ T cell biology in the mouse system, leveraging 53-5.8 as a key reagent for identifying, depleting, or functionally analyzing CD8?-expressing cells. Key findings from scientific literature citing clone 53-5.8, an anti-mouse CD8? monoclonal antibody, include its effectiveness for depleting CD8+ T cells in mice, the distinct effects it has on immune cell populations compared to anti-CD8? antibodies, and its utility in dissecting CD8+ T-cell–mediated immune functions and pathologies. Essential findings and context from major citations:
Summary Table: Clone 53-5.8 vs. Anti-CD8? (53-6.7)
Additional Notes:
In summary, the scientific literature characterizes clone 53-5.8 as a potent, widely used tool for mouse CD8+ T-cell depletion that profoundly influences subsequent immune responses and experimental outcomes. References & CitationsTechnical ProtocolsCertificate of Analysis |
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