Anti-Mouse EpCAM (CD326) [Clone G8.8] — Purified in vivo GOLD^TM Functional Grade

Clone G8.8 is a rat monoclonal antibody specific to mouse EpCAM (CD326) that is widely used in in vivo mouse studies primarily to identify, isolate, and study epithelial cells and thymic epithelial cells.

Key uses in in vivo mouse studies include:

• Flow cytometry (FACS): G8.8 is used for isolating and quantifying EpCAM-expressing cells from mouse tissues, such as in sorting epithelial cells or thymic epithelial cells for downstream analysis or transplantation studies.
• Immunohistochemistry (IHC) and immunofluorescence (IF): The antibody is employed to stain tissue sections from mouse organs to localize and study the distribution and phenotype of EpCAM+ cells, especially in epithelial compartments. A typical working concentration is 2–5?µg/ml for IHC, IF, and ICC.
• Immunoprecipitation and Western blot: G8.8 can be used for protein analysis, confirming the presence and amount of EpCAM in mouse tissues.
• Cell purification: It has been specifically cited for the purification of thymic epithelial cells in mice (e.g., PMID: 20548034, 17127749).

Additional essential details:

• Specificity: G8.8 binds specifically to mouse EpCAM and does not cross-react with human or rat EpCAM.
• Immunogen: The antibody was raised against the TE-71 mouse thymic epithelial cell line, using the full-length native EpCAM protein as immunogen.

In summary, clone G8.8 is a standard reagent for marking, isolating, and studying mouse epithelial and thymic epithelial cell populations in vivo and ex vivo across a variety of experimental applications.

I don't have specific information about the storage temperature requirements for sterile packaged clone G8.8 in the provided search results. The search results contain general storage guidelines for various biological materials and laboratory reagents, but none specifically mention clone G8.8 or its storage requirements.

The search results do provide storage temperatures for other laboratory materials: plasmid vectors are typically stored at -20°C, virus stocks require -80°C storage, and certain enzyme mixes can be stored at -20°C for short term (6 months) or -80°C for long-term storage. However, without specific information about clone G8.8 - including what type of biological material it is, how it's packaged, and manufacturer specifications - I cannot determine the correct storage temperature.

For accurate storage requirements for clone G8.8, you should consult the manufacturer's documentation, product insert, or contact the supplier directly, as storage conditions can vary significantly depending on the specific type of clone, its formulation, and packaging.

The G8.8 antibody is most commonly used to detect EpCAM (CD326), and in the literature, it is frequently employed alongside several other antibodies and proteins depending on the specific research context.

Commonly used antibodies/proteins with G8.8:

• Other anti-EpCAM antibodies: Researchers sometimes co-use different EpCAM antibodies targeting alternate epitopes to confirm specificity or for cross-comparison in assays.
• Immune cell markers: For studies on cell populations or immunophenotyping, antibodies against CD3, CD45, CD4, CD8, and CD19 are frequently used with G8.8 to identify and characterize various lymphocyte subsets in tissue, especially in mucosal immunology or cancer studies.
• Epithelial and tumor markers: Cytokeratin antibodies (e.g., CK8, CK18), E-cadherin, or pan-cytokeratin are often paired with G8.8 to define epithelial cells, assess cell adhesion, or analyze carcinoma samples.
• Antibodies for functional or co-localization studies: In immunofluorescence or flow cytometry, antibodies like anti-?-catenin, anti-MHC molecules, or anti-CD44 may be used to study interactions, signaling, or cell localization in combination with G8.8.
• Fusion proteins and conjugates: Researchers sometimes fuse antibodies with enzymes (e.g., alkaline phosphatase, luciferase), tags, or reporter constructs for detection—these proteins may be co-applied with G8.8 in assays such as ELISA or imaging.

Examples from literature:

• In epithelial cell or cancer research, G8.8 is commonly paired with immunofluorescent markers for other cell surface proteins for co-localization or comparative expression studies.
• In immune interaction studies (e.g., with intraepithelial lymphocytes), antibodies against lymphocyte markers (CD3, CD8) are typically included in the experimental setup.
• In bioconjugation or ELISA methods, enzyme-labeled antibodies or fusion proteins can be produced and used alongside G8.8 for multiplex detection.

In summary, the most common companion antibodies or proteins used with G8.8 in published research are immune cell markers, other epithelial cell markers, and enzyme or reporter conjugates—with the specific choices tailored to the experimental goals and techniques used in each study.

Clone G8.8 is a well-established rat monoclonal antibody that targets the mouse epithelial cell adhesion molecule (EpCAM/CD326), and its citations in scientific literature have contributed to several important findings across multiple research areas.

Primary Antibody Characteristics and Applications

The G8.8 clone was deposited to the Developmental Studies Hybridoma Bank by Farr, A.G. from the University of Washington in 1997. This antibody specifically recognizes mouse EpCAM but notably does not cross-react with human or rat EpCAM, making it highly species-specific. The antibody has proven versatile across multiple experimental techniques, with confirmed applications including flow cytometry (FACS), immunofluorescence, immunohistochemistry, immunoprecipitation, and Western blotting.

Thymic Epithelial Cell Research

One of the most significant applications of G8.8 has been in thymic epithelial cell purification and characterization. The antibody has been successfully used to isolate and purify thymic epithelial cells, which are crucial components of the thymic microenvironment responsible for T cell development and selection. This application has been particularly valuable for researchers studying immune system development and thymic function.

Cancer Research Applications

Recent citations demonstrate G8.8's utility in cancer-associated fibroblast (CAF) research, particularly in pancreatic ductal adenocarcinoma (PDAC) studies. The antibody has been employed in cross-species single-cell analyses that revealed important insights about antigen-presenting cancer-associated fibroblasts. These studies utilized G8.8 in conjunction with other markers to characterize different CAF subtypes and their roles in tumor microenvironments.

Immunological Function Studies

The literature shows that G8.8 has contributed to understanding antigen presentation mechanisms in cancer contexts. Research using this antibody has helped identify CAF populations capable of presenting antigens to CD4+ T cells, demonstrating that certain cancer-associated fibroblasts can function as non-professional antigen-presenting cells. These findings have implications for understanding how tumors modulate immune responses.

Technical Methodological Contributions

Citations of G8.8 have established important methodological protocols for EpCAM-based cell sorting and analysis. The antibody's reliable performance in flow cytometry applications has made it a standard tool for identifying and isolating EpCAM-positive cell populations in mouse models. Its use in immunohistochemistry applications, particularly with acetone-fixed frozen sections, has provided researchers with consistent staining protocols.

The extensive citation history of clone G8.8 reflects its value as a research tool that has enabled discoveries spanning developmental immunology, cancer biology, and cellular characterization methodologies, establishing it as a reliable reagent for mouse EpCAM detection across diverse experimental contexts.