Anti-Mouse H-2Db (Clone B22/249) – Purified In Vivo Antibody

Anti-Mouse H-2Db (Clone B22/249) – Purified in vivo PLATINUM™ Functional Grade

Product No.: B341


Clone B22/249 Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names H-2D; H2-D; H2-D1 Isotype Mouse IgG2a k Applications Cytotoxicity Assay , ELISA , FA , FC , IF , In vitro depletion , IP


Select Product Size 100 mg — $3,300.00 25 mg — $1,550.00 50 mg — $2,300.00 5.0 mg — $455.00 1.0 mg — $240.00	Qty Max: Min: 1 Step: 1 Select A Size ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Mouse Host Species Mouse Recommended Isotype Controls Mouse IgG2a PLATINUM Recommended Dilution Buffer In vivo Antibody Diluent pH 7.2 Immunogen Mouse H-2Db Product Concentration ≥ 5.0 mg/ml Endotoxin Level ≤ 0.5 EU/mg as determined by the LAL method Purity ≥95% by SDS Page ⋅ ≥98% monomer by analytical SEC Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. State of Matter Liquid Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM<sup>TM</sup> antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Regulatory Status Research Use Only Country of Origin USA Shipping 2-8°C Wet Ice Additional Applications Reported In Literature ? In vitro depletion, Cytotoxicity Assay, IP, FC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity B22.249.R1 activity is directed against mouse H-2Db. Background H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response¹. H-2Db is a class I MHC cell surface protein. MHC class I molecules bind peptides generated by the degradation of cytosolic proteins, and then display those peptides on the cell surface. Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation. For example, H-2Db plays a role in human papillomavirus infection². When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell¹. B22-249.R1 was generated by immunizing mice with spleen cells from allogeneic mice using the combination BSLB/k anti-C57BL/6³. Spleen cells of the recipient mouse were hybridized with the P3-NS 1-Ag4 myeloma cell line and screened using cytotoxic and hemagglutination assays. B22-249.R1 is also known as H-2.m2 in the literature⁴. Antigen Distribution H-2Db is expressed on the cell surface of lymphocytes. Ligand/Receptor CD3/TCR, CD8 NCBI Gene Bank ID 14964 UniProt.org P01899 Research Area Immunology Leinco Antibody Advisor Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. The B22/249 clone is a monoclonal antibody that targets the mouse H-2Db MHC class I molecule and has several important applications in in vivo mouse studies. Target and Mechanism This antibody specifically binds to H-2Db, which is expressed on the cell surface of lymphocytes. The H-2Db molecule is part of the major histocompatibility complex (MHC) class I system and interacts with CD3/TCR and CD8 receptors. When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell. Primary Applications in Research The B22/249 clone serves multiple functions in in vivo mouse studies: Cytotoxicity Assays: The antibody is used to study cytotoxic T cell responses by targeting H-2Db-expressing cells. This allows researchers to investigate how immune cells recognize and eliminate target cells. In Vitro Depletion Studies: Researchers use this antibody to selectively deplete H-2Db-expressing cells from cell populations before transplantation or other experimental procedures. Immunoprecipitation (IP): The antibody can isolate H-2Db complexes for further analysis of MHC class I presentation mechanisms. Technical Specifications for In Vivo Use The antibody is specifically formulated for in vivo applications with low endotoxin levels (? 1.0 EU/mg), which is crucial for preventing inflammatory responses that could confound experimental results. It's manufactured as a functional-grade preclinical antibody using animal-free in vitro cell culture techniques and purified through multiple steps to ensure extremely low levels of contaminants. The B22/249 clone is a mouse IgG2a ? isotype antibody that maintains high purity (?95% by SDS Page and ?95% monomer by analytical SEC). This high purity is essential for reproducible results in sensitive in vivo experiments where antibody aggregates or contaminants could interfere with the study outcomes. Research Context This antibody is particularly valuable in cancer immunology and immunology research, where understanding MHC class I presentation and T cell responses is critical for developing immunotherapeutic approaches and studying immune system function in mouse models. The correct storage temperature for sterile packaged clone B22/249 is 2–8°C if you are storing it as received for up to one month. For long-term storage, you should aliquot the antibody under aseptic conditions and store it at ? –70°C to maintain stability. Short-term (as received, sterile): 2–8°C (up to one month). Long-term: Aliquot and store at ? –70°C. Avoid repeated freeze-thaw cycles. Always consult the lot-specific datasheet for any special requirements, as protocols may periodically change. B22/249 is a mouse monoclonal antibody that specifically targets H-2Db, a mouse MHC class I molecule used in immunological studies such as cytotoxicity assays, in vitro cell depletion, and flow cytometry. When B22/249 is used in literature, it is often combined with additional antibodies or proteins to characterize mouse immune cell subsets or the immunological environment. Commonly used antibodies/proteins found alongside B22/249 include: Antibodies for other MHC class I molecules: H-2Kb is frequently studied in parallel with H-2Db to distinguish between different MHC class I isoforms on murine cells. Clones such as AF6-88.5 (H-2Kb) are routinely used in combination. Antibodies for MHC class II molecules: H-2 I-A/I-E isotypes (e.g., clone M5/114.15.2) may be used to characterize antigen-presenting cells or distinguish between class I and class II MHC expression. Immune lineage and activation markers: CD3, CD8, and CD4 are typical T cell markers applied in multi-color flow cytometry with H-2Db to determine T cell subsets recognizing presented antigens. CD45, CD19, NK1.1, and other immune lineage markers are also often applied, depending on the experimental focus. Other MHC class I antibodies: Alternative anti-H-2Db clones (such as 28-14-8S, AG76, OASB00706) are available and sometimes used for comparison or in multi-color assays. Functional reagents: Tetramers or multimers of MHC class I loaded with specific peptides are often paired with B22/249 to track antigen-specific T cells. Proteins and reagents for detection: Secondary antibodies (e.g., anti-mouse IgG), protein G, and detection tags (biotin, FITC, PE) are used for visualization in flow cytometry and immunoprecipitation. Specific combinations will depend on the research question (e.g., measuring CD8+ T cell responses, studying antigen presentation, or depleting particular lymphocyte populations), but the above list covers the most common types used alongside B22/249 in immunological studies. Clone B22/249 is a monoclonal antibody targeting the mouse H-2Db molecule, which is a major histocompatibility complex (MHC) class I molecule expressed on the surface of lymphocytes. This antibody has been used in various immunological studies, often related to cancer research and immune system modulation. Here are some key findings or applications associated with clone B22/249 in scientific literature: In Vivo and In Vitro Applications: Clone B22/249 is used for in vivo applications, such as depleting H-2Db-expressing cells, and in vitro techniques like cytotoxicity assays and immunoprecipitation (IP) . Cancer Research: The antibody is utilized in cancer studies to modulate immune responses. For example, it might be used to study the effects of MHC class I depletion on tumor immunity . Immunological Studies: Clone B22/249 is employed in research focused on understanding the role of MHC class I molecules in immune cell function and interactions. This includes studies on T cell recognition and antigen presentation . Gene Editing and Knockout Studies: While not directly related to B22/249, the context of its use involves gene editing techniques like CRISPR/Cas9 to modify genes involved in immune responses, such as B2m (beta-2 microglobulin), which is crucial for MHC class I expression . Overall, clone B22/249 is an important tool in immunological research, especially in studies involving MHC class I molecules and their role in immune responses. References & Citations 1. Yoshida R. Adv Immunol. 124:207-247. 2014. 2. Peng S, Mattox A, Best SR, et al. Cancer Immunol Immunother. 65(3):261-271. 2016. 3. Hammerling GJ, Hammerling U, and Lemke HL. Immunogenetics. 8:433. 1979. 4. Klein J, Huang HJS, Lemke H, et al. Immunogenetics. 8:419. 1979. 5. Maloy WL, Hämmerling G, Nathenson SG, et al. J Immunol Methods. 37(3-4):287-299. 1980. 6. Maloy WL, Coligan JE. Immunogenetics. 16(1):11-22. 1982. 7. Melino M, Nichols E, Strausser H, et al. J lmmunol. 129:222. 1982. 8. Shapiro LH, Dugan ES, Neiderhuber JE. J Exp Med. 162(5):1477-1493. 1985. View product citations for antibody B341 on CiteAb Technical Protocols Cytotoxicity Assay FA IF In vitro depletion Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Mouse

Recommended Isotype Controls

Mouse IgG2a PLATINUM

Recommended Dilution Buffer

In vivo Antibody Diluent pH 7.2

Immunogen

Mouse H-2Db

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 0.5 EU/mg as determined by the LAL method

Purity

≥95% by SDS Page

⋅

≥98% monomer by analytical SEC

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

State of Matter

Liquid

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Pathogen Testing

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM<sup>TM</sup> antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Regulatory Status

Research Use Only

Country of Origin

USA

Shipping

2-8°C Wet Ice

Additional Applications Reported In Literature ?

In vitro depletion,
Cytotoxicity Assay,
IP,
FC

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

B22.249.R1 activity is directed against mouse H-2Db.

Background

H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response¹. H-2Db is a class I MHC cell surface protein. MHC class I molecules bind peptides generated by the degradation of cytosolic proteins, and then display those peptides on the cell surface. Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation. For example, H-2Db plays a role in human papillomavirus infection². When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell¹.

B22-249.R1 was generated by immunizing mice with spleen cells from allogeneic mice using the combination BSLB/k anti-C57BL/6³. Spleen cells of the recipient mouse were hybridized with the P3-NS 1-Ag4 myeloma cell line and screened using cytotoxic and hemagglutination assays. B22-249.R1 is also known as H-2.m2 in the literature⁴.

Antigen Distribution

H-2Db is expressed on the cell surface of lymphocytes.

Ligand/Receptor

CD3/TCR, CD8

NCBI Gene Bank ID

14964

UniProt.org

P01899

Research Area

Immunology

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

How is this clone B22/249 used in in vivo mouse studies? +

The B22/249 clone is a monoclonal antibody that targets the mouse H-2Db MHC class I molecule and has several important applications in in vivo mouse studies.

Target and Mechanism

This antibody specifically binds to H-2Db, which is expressed on the cell surface of lymphocytes. The H-2Db molecule is part of the major histocompatibility complex (MHC) class I system and interacts with CD3/TCR and CD8 receptors. When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell.

Primary Applications in Research

The B22/249 clone serves multiple functions in in vivo mouse studies:

Cytotoxicity Assays: The antibody is used to study cytotoxic T cell responses by targeting H-2Db-expressing cells. This allows researchers to investigate how immune cells recognize and eliminate target cells.

In Vitro Depletion Studies: Researchers use this antibody to selectively deplete H-2Db-expressing cells from cell populations before transplantation or other experimental procedures.

Immunoprecipitation (IP): The antibody can isolate H-2Db complexes for further analysis of MHC class I presentation mechanisms.

Technical Specifications for In Vivo Use

The antibody is specifically formulated for in vivo applications with low endotoxin levels (? 1.0 EU/mg), which is crucial for preventing inflammatory responses that could confound experimental results. It's manufactured as a functional-grade preclinical antibody using animal-free in vitro cell culture techniques and purified through multiple steps to ensure extremely low levels of contaminants.

The B22/249 clone is a mouse IgG2a ? isotype antibody that maintains high purity (?95% by SDS Page and ?95% monomer by analytical SEC). This high purity is essential for reproducible results in sensitive in vivo experiments where antibody aggregates or contaminants could interfere with the study outcomes.

Research Context

This antibody is particularly valuable in cancer immunology and immunology research, where understanding MHC class I presentation and T cell responses is critical for developing immunotherapeutic approaches and studying immune system function in mouse models.

What is the correct storage temperature for this sterile packaged clone B22/249? +

The correct storage temperature for sterile packaged clone B22/249 is 2–8°C if you are storing it as received for up to one month. For long-term storage, you should aliquot the antibody under aseptic conditions and store it at ? –70°C to maintain stability.

Short-term (as received, sterile): 2–8°C (up to one month).
Long-term: Aliquot and store at ? –70°C. Avoid repeated freeze-thaw cycles.

Always consult the lot-specific datasheet for any special requirements, as protocols may periodically change.

What are some of the other commonly used antibodies or proteins used with B22/249 in the literature? +

B22/249 is a mouse monoclonal antibody that specifically targets H-2Db, a mouse MHC class I molecule used in immunological studies such as cytotoxicity assays, in vitro cell depletion, and flow cytometry. When B22/249 is used in literature, it is often combined with additional antibodies or proteins to characterize mouse immune cell subsets or the immunological environment.

Commonly used antibodies/proteins found alongside B22/249 include:

Antibodies for other MHC class I molecules:
- H-2Kb is frequently studied in parallel with H-2Db to distinguish between different MHC class I isoforms on murine cells. Clones such as AF6-88.5 (H-2Kb) are routinely used in combination.
Antibodies for MHC class II molecules:
- H-2 I-A/I-E isotypes (e.g., clone M5/114.15.2) may be used to characterize antigen-presenting cells or distinguish between class I and class II MHC expression.
Immune lineage and activation markers:
- CD3, CD8, and CD4 are typical T cell markers applied in multi-color flow cytometry with H-2Db to determine T cell subsets recognizing presented antigens.
- CD45, CD19, NK1.1, and other immune lineage markers are also often applied, depending on the experimental focus.
Other MHC class I antibodies:
- Alternative anti-H-2Db clones (such as 28-14-8S, AG76, OASB00706) are available and sometimes used for comparison or in multi-color assays.
Functional reagents:
- Tetramers or multimers of MHC class I loaded with specific peptides are often paired with B22/249 to track antigen-specific T cells.
Proteins and reagents for detection:
- Secondary antibodies (e.g., anti-mouse IgG), protein G, and detection tags (biotin, FITC, PE) are used for visualization in flow cytometry and immunoprecipitation.

Specific combinations will depend on the research question (e.g., measuring CD8+ T cell responses, studying antigen presentation, or depleting particular lymphocyte populations), but the above list covers the most common types used alongside B22/249 in immunological studies.

What are the key findings from clone B22/249 citations in scientific literature? +

Clone B22/249 is a monoclonal antibody targeting the mouse H-2Db molecule, which is a major histocompatibility complex (MHC) class I molecule expressed on the surface of lymphocytes. This antibody has been used in various immunological studies, often related to cancer research and immune system modulation. Here are some key findings or applications associated with clone B22/249 in scientific literature:

In Vivo and In Vitro Applications: Clone B22/249 is used for in vivo applications, such as depleting H-2Db-expressing cells, and in vitro techniques like cytotoxicity assays and immunoprecipitation (IP) .
Cancer Research: The antibody is utilized in cancer studies to modulate immune responses. For example, it might be used to study the effects of MHC class I depletion on tumor immunity .
Immunological Studies: Clone B22/249 is employed in research focused on understanding the role of MHC class I molecules in immune cell function and interactions. This includes studies on T cell recognition and antigen presentation .
Gene Editing and Knockout Studies: While not directly related to B22/249, the context of its use involves gene editing techniques like CRISPR/Cas9 to modify genes involved in immune responses, such as B2m (beta-2 microglobulin), which is crucial for MHC class I expression .

Overall, clone B22/249 is an important tool in immunological research, especially in studies involving MHC class I molecules and their role in immune responses.

References & Citations

1. Yoshida R. Adv Immunol. 124:207-247. 2014.
2. Peng S, Mattox A, Best SR, et al. Cancer Immunol Immunother. 65(3):261-271. 2016.
3. Hammerling GJ, Hammerling U, and Lemke HL. Immunogenetics. 8:433. 1979.
4. Klein J, Huang HJS, Lemke H, et al. Immunogenetics. 8:419. 1979.
5. Maloy WL, Hämmerling G, Nathenson SG, et al. J Immunol Methods. 37(3-4):287-299. 1980.
6. Maloy WL, Coligan JE. Immunogenetics. 16(1):11-22. 1982.
7. Melino M, Nichols E, Strausser H, et al. J lmmunol. 129:222. 1982.
8. Shapiro LH, Dugan ES, Neiderhuber JE. J Exp Med. 162(5):1477-1493. 1985.

View product citations for antibody B341 on CiteAb

Cytotoxicity Assay

In vitro depletion

Certificate of Analysis

Request COA

Formats Available

Prod No.	Description
B344	Anti-Mouse H-2Db (Clone B22/249) – FITC
B345	Anti-Mouse H-2Db (Clone B22/249) – Biotin
B342	Anti-Mouse H-2Db (Clone B22/249) – APC
B343	Anti-Mouse H-2Db (Clone B22/249) – PE
B340	Anti-Mouse H-2Db (Clone B22/249) – Purified in vivo GOLD™ Functional Grade
B346	Anti-Mouse H-2Db (Clone B22/249) – Dylight® 488
B347	Anti-Mouse H-2Db (Clone B22/249) – Dylight® 550
B348	Anti-Mouse H-2Db (Clone B22/249) – Dylight® 650
B349	Anti-Mouse H-2Db (Clone B22/249) – Dylight® 594
B341	Anti-Mouse H-2Db (Clone B22/249) – Purified in vivo PLATINUM™ Functional Grade

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

NEW Products!

Anti-Mouse H-2Db (Clone B22/249) – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse H-2Db (Clone B22/249) – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse H-2Db (Clone B22/249) – Purified in vivo PLATINUM™ Functional Grade

Antibody Details

Product Details

Description

Description

Leinco Antibody Advisor

Target and Mechanism

Primary Applications in Research

Technical Specifications for In Vivo Use

Research Context

References & Citations

Certificate of Analysis

Formats Available

Subscribe to Our Newsletter

Products

Custom Services

About Us

Support

NEW Products!

Anti-Mouse H-2Db (Clone B22/249) – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse H-2Db (Clone B22/249) – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse H-2Db (Clone B22/249) – Purified in vivo PLATINUM™ Functional Grade

Antibody Details

Product Details

Description

Description

Leinco Antibody Advisor

Target and Mechanism

Primary Applications in Research

Technical Specifications for In Vivo Use

Research Context

References & Citations

Technical Protocols

Certificate of Analysis

Formats Available

Subscribe to Our Newsletter

Products

Custom Services

About Us

Support