Anti-Mouse H-2Db (Clone B22/249) – Purified in vivo GOLD™ Functional Grade

Anti-Mouse H-2Db (Clone B22/249) – Purified in vivo GOLD™ Functional Grade

Product No.: B340

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Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Alternate Names
H-2D; H2-D; H2-D1
Mouse IgG2a k
Cytotoxicity Assay
In vitro depletion

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Antibody Details

Product Details

Reactive Species
Host Species
Mouse H-2Db
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
≥95% by SDS Page
≥95% monomer by analytical SEC
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
2-8°C Wet Ice
Additional Applications Reported In Literature ?
In vitro depletion,
Cytotoxicity Assay,
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


B22.249.R1 activity is directed against mouse H-2Db.
Antigen Distribution
H-2Db is expressed on the cell surface of lymphocytes.
H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response1. H-2Db is a class I MHC cell surface protein. MHC class I molecules bind peptides generated by the degradation of cytosolic proteins, and then display those peptides on the cell surface. Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation. For example, H-2Db plays a role in human papillomavirus infection2. When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell1.

B22-249.R1 was generated by immunizing mice with spleen cells from allogeneic mice using the combination BSLB/k anti-C57BL/63. Spleen cells of the recipient mouse were hybridized with the P3-NS 1-Ag4 myeloma cell line and screened using cytotoxic and hemagglutination assays. B22-249.R1 is also known as H-2.m2 in the literature4.

Antigen Details

NCBI Gene Bank ID
Research Area

References & Citations

1. Yoshida R. Adv Immunol. 124:207-247. 2014.
2. Peng S, Mattox A, Best SR, et al. Cancer Immunol Immunother. 65(3):261-271. 2016.
3. Hammerling GJ, Hammerling U, and Lemke HL. Immunogenetics. 8:433. 1979.
4. Klein J, Huang HJS, Lemke H, et al. Immunogenetics. 8:419. 1979.
5. Maloy WL, Hämmerling G, Nathenson SG, et al. J Immunol Methods. 37(3-4):287-299. 1980.
6. Maloy WL, Coligan JE. Immunogenetics. 16(1):11-22. 1982.
7. Melino M, Nichols E, Strausser H, et al. J lmmunol. 129:222. 1982.
8. Shapiro LH, Dugan ES, Neiderhuber JE. J Exp Med. 162(5):1477-1493. 1985.
Cytotoxicity Assay
Indirect Elisa Protocol
Flow Cytometry
In vitro depletion
Immunoprecipitation Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.