Anti-Mouse I-A/I-E (MHC Class II) [Clone M5/114.15.2] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse I-A/I-E (MHC Class II) [Clone M5/114.15.2] — Purified in vivo GOLD™ Functional Grade
Product No.: H271
Clone M5/114.15.2 Target I-A/I-E MHC class II Formats AvailableView All Product Type Monoclonal Antibody Isotype Rat IgG2b Applications B , FC , IHC FF , in vivo , IP , PhenoCycler® |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen C57BL/6 Spleen Cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2892895 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this Clone M5/114.15.2 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IHC FF B IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone M5/114.15.2 recognizes an epitope on the mouse MHC class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. Clone M5/114.15.2 does not react with I-Af, I-Ak, or I-As.
Background MHC class II antibody, clone M5/114.15.2, recognizes the major histocompatibility complex (MHC) class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. MHC class II is constitutively expressed on professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation1. MHC class II consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain2. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens3. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling4. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation5 and can transduce signals into T cells, enhancing T cell proliferation and activity6. Antigen Distribution I-Ab/d/q and I-Ed/k are expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells from mice of the I-Ab/d/q and I-Ed/k haplotypes. Ligand/Receptor CD3/TCR, CD4 PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology . Innate Immunity Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone M5/114.15.2 is a rat IgG2b monoclonal antibody used in in vivo mouse studies primarily to target and block MHC class II molecules (I-A and I-E) present on antigen-presenting cells (APCs) such as macrophages, dendritic cells, B cells, and activated T cells. Key uses in in vivo mouse studies:
Mechanism:
Strain specificity:
Preparation and handling:
Additional information:
In summary, in in vivo mouse studies, M5/114.15.2 is used as a functional blocker of MHC class II to manipulate antigen presentation and CD4+ T cell responses, offering a powerful tool for dissecting adaptive immune mechanisms. Commonly used antibodies and proteins alongside M5/114.15.2 (a rat monoclonal antibody against mouse MHC class II I-A/I-E) in the literature primarily target markers that identify or characterize the same professional antigen-presenting cells, lymphocyte populations, or their activation states. These pairings support applications such as flow cytometry, immunohistochemistry, and immune profiling. Frequently co-used antibodies and proteins include:
These combinations allow for detailed characterization of immune cell subsets, especially in mouse immunology research, leveraging the specificity of M5/114.15.2 for MHC II (I-A/I-E) molecules that define antigen-presenting cells. Additionally, when performing functional assays or immunohistochemistry, M5/114.15.2 may be used with:
This panel strategy enables discrimination of cell types, maturation stages, and activation states in immunological studies, consistent with the broad applications of M5/114.15.2 outlined in standard protocols. Clone M5/114.15.2 is a rat monoclonal antibody widely cited in scientific literature for its specificity to mouse MHC class II molecules (I-A and I-E subregions), making it a key tool in immunology for detecting, quantifying, and depleting cells expressing these molecules. Key findings and uses from scientific literature include:
The consistent finding across scientific literature is that M5/114.15.2 is a foundational tool for dissecting MHC class II immunobiology in murine models and for experimental manipulation of APCs in studies of immune responses, tolerance, and autoimmunity. Dosing regimens of the monoclonal antibody clone M5/114.15.2 vary by application, mouse model, and experimental aim, but are most commonly dictated empirically based on the context of use, such as flow cytometry, in vitro/in vivo blocking, or depletion. For in vitro applications (e.g., flow cytometry), Thermo Fisher reports:
For in vivo use, published dosing regimens in the literature for M5/114.15.2 vary because they are tailored to the immune cell population targeted, mouse strain (e.g., C57BL/6, NOD, BALB/c), route of administration, and the intended immunological effect (depletion, blockade, or tracking). Key points from reported regimens:
No direct citation from your results provides exhaustive detail on in vivo dosing across all models, but general guidance universally emphasizes careful titration and pilot studies to optimize for each experimental context. Summary Table: Typical Ranges (for reference)
Key variables affecting regimen:
For precise guidance, researchers universally recommend pilot dose-response studies to calibrate for:
If you require dosing regimens for specific published mouse model studies using M5/114.15.2, searching primary literature or supplier protocols (often found in supplementary methods) will yield model- and endpoint-specific guidance, as supplier datasheets do not differentiate by mouse strain. References & Citations1. Holling TM, et al. (2004) Hum Immunol. 65(4):282-90 2. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):10618-25 3. Wieczorek M, et al. (2017) Front Immunol. 8:292 4. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):16916-16921 5. Barnaba V, et al. (1994) Eur J Immunol. 24(1):71-5 6. Di Rosa F, et al. (1993) Hum Immunol. 38(4):251-60 Technical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
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H502 | |
H271 | |
H1721 | |
H1678 | |
H253 | |
H273 | |
H272 | |
H1720 | |
H1722 | |
H274 | |
H270 |
