Anti-Mouse I-A/I-E (MHC Class II) – DyLight® 594

Anti-Mouse I-A/I-E (MHC Class II) – DyLight® 594

Product No.: H1721

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Clone
M5/114.15.2
Target
I-A/I-E
MHC class II
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG2b
Applications
FC
,
IHC FF

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
C57BL/6 Spleen Cells
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 594 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 594 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Red Laser (590 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this Clone M5/114.15.2 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
IHC FF
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone M5/114.15.2 recognizes an epitope on the mouse MHC class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. Clone M5/114.15.2 does not react with I-Af, I-Ak, or I-As.
Antigen Distribution
I-Ab/d/q and I-Ed/k are expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells from mice of the I-Ab/d/q and I-Ed/k haplotypes.
Background
MHC class II antibody, clone M5/114.15.2, recognizes the major histocompatibility complex (MHC) class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. MHC class II is constitutively expressed on professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation1. MHC class II consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain2. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens3. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling4. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation5 and can transduce signals into T cells, enhancing T cell proliferation and activity6.

Antigen Details

Ligand/Receptor
CD3/TCR, CD4
PubMed
NCBI Gene Bank ID
Research Area
Immunology
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Innate Immunity

References & Citations

1. Holling TM, et al. (2004) Hum Immunol. 65(4):282-90
2. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):10618-25
3. Wieczorek M, et al. (2017) Front Immunol. 8:292
4. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):16916-16921
5. Barnaba V, et al. (1994) Eur J Immunol. 24(1):71-5
6. Di Rosa F, et al. (1993) Hum Immunol. 38(4):251-60
Flow Cytometry
IHC FF
Products are for research use only. Not for use in diagnostic or therapeutic procedures.