Anti-Mouse ICAD (CT)

Pricing & Details

Product No.I-299
Protein
ICAD
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
DFF45, Inhibitor of Caspase-Activated DNASE (CAD)
Applications
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-299-20ug
20 µg
$195.00
In stock
Max:
Min: 1
Step: 1
I-299-0.1 mg
0.1 mg
$397.00
In stock
Max:
Min: 1
Step: 1

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rabbit
Immunogen
PN:I-311
Product Concentration
0.5 mg/ml
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: This ICAD antibody can be used for detection of of ICAD by Western blot at 1:1000 dilution. A 45 kDa band can be detected.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Mouse ICAD recognizes an epitope near the C-terminus of mouse ICAD. This polyclonal antibody was purified using affinity chromatography.
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Liu, X. et al. (1997) Cell 89:175-184
2. Enari, M. et al. (1998) Nature 391:43-50
3. Sakahira, H. et al. (1998) Nature 391:96-99
4. Wyllie, A. (1998) Nature 391(6662):20-1.
General Western Blot Protocol
Prod No.
Description
I-299
I-312
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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