Anti-Mouse ICAD (NT)

Anti-Mouse ICAD (NT)

Product No.: I-312

[product_table name="All Top" skus="I-312"]

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Target
ICAD
Product Type
Polyclonal Antibody
Alternate Names
DFF45, Inhibitor of Caspase-Activated DNASE
Applications
IHC FFPE
,
WB

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Select Product Size

Data

I-312 Western Blot
I-312 IHC Murine Lung Tissue
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rabbit
Immunogen
PN:I-326
Product Concentration
0.5 mg/ml
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Rabbit Anti-Mouse ICAD recognizes an epitope near the N-terminus of Mouse ICAD. This polyclonal antibody was purified using affinity chromatography.
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which is cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.
PubMed

References & Citations

1. Liu, X. et al. (1997) Cell 89:175-184
2. Enari, M. et al. (1998) Nature 391:43-50
3. Sakahira, H. et al. (1998) Nature 391:96-99
4. Wyllie, A. (1998) Nature 391(6662):20-1.
IHC FFPE
General Western Blot Protocol

Certificate of Analysis

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Prod No.
Description
I-299
I-312
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.