Anti-Mouse IL-3 Rβ (Clone 130710)

Product No.: I-679


Clone 130710 Target IL-3 R beta Formats AvailableView All Product Type Monoclonal Antibody Alternate Names AIC2A, CD131, Interleukin-3 Receptor Beta Isotype IgG2a Applications ELISA Cap


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Antibody Details Product Details Reactive Species Mouse Host Species Rat Immunogen NS0-Derived Recombinant Mouse Interleukin-3 Receptor β Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830800 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 4 µg/ml. A suitable detection antibody is PN:I-784 at a concentration of approximately 100 µg/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is 8 ng/well. A suggested standard for this assay is PN:I-568. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Rat Anti-Mouse Interleukin 3 Receptor Beta (IL-3 Rβ) (Clone 130710) recognizes an epitope on Mouse IL-3 Rβ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. No cross-reactivity or interference was observed with rmIL-3 Rα or rhIL-3 Rα. Cross-reactivity with AIC2B was not tested PubMed IL-3 R beta NCBI Gene Bank ID 16187 UniProt.org Information on Uniprot.org References & Citations View product citations for antibody I-679 on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Rat

Immunogen

NS0-Derived Recombinant Mouse Interleukin-3 Receptor β Extracellular Domain

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830800

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 4 µg/ml. A suitable detection antibody is PN:I-784 at a concentration of approximately 100 µg/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is 8 ng/well. A suggested standard for this assay is PN:I-568.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse Interleukin 3 Receptor Beta (IL-3 Rβ) (Clone 130710) recognizes an epitope on Mouse IL-3 Rβ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. No cross-reactivity or interference was observed with rmIL-3 Rα or rhIL-3 Rα. Cross-reactivity with AIC2B was not tested

PubMed

IL-3 R beta

NCBI Gene Bank ID

16187

UniProt.org

Information on Uniprot.org