Anti-Mouse IL-3 Rβ (Clone 130710)

Anti-Mouse IL-3 Rβ (Clone 130710)

Product No.: I-679

[product_table name="All Top" skus="I-679"]

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Clone
130710
Target
IL-3 R beta
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
AIC2A, CD131, Interleukin-3 Receptor Beta
Isotype
IgG2a
Applications
ELISA Cap

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
NS0-Derived Recombinant Mouse Interleukin-3 Receptor β Extracellular Domain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 4 µg/ml. A suitable detection antibody is PN:I-784 at a concentration of approximately 100 µg/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is 8 ng/well. A suggested standard for this assay is PN:I-568.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Interleukin 3 Receptor Beta (IL-3 Rβ) (Clone 130710) recognizes an epitope on Mouse IL-3 Rβ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. No cross-reactivity or interference was observed with rmIL-3 Rα or rhIL-3 Rα. Cross-reactivity with AIC2B was not tested
NCBI Gene Bank ID

References & Citations

Elisa Sandwich Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
I-679
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.